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Article

How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights

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SC Scienze e Tecnologie Chirurgiche-SS Piattaforma Scienze Omiche per Ortopedia Personalizzata, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy
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IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy
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Department of Biomedicine, Neuroscience and Advanced Diagnostics (Bi.N.D.), Section of Biology and Genetics, University of Palermo, 90133 Palermo, Italy
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Istituto per la Ricerca e l’Innovazione Biomedica (IRIB), 90133 Palermo, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Susanne Grässel
Int. J. Mol. Sci. 2021, 22(5), 2471; https://doi.org/10.3390/ijms22052471
Received: 15 January 2021 / Revised: 24 February 2021 / Accepted: 24 February 2021 / Published: 28 February 2021
Osteoarthritis (OA) is a degenerative bone disease that involved micro and macro-environment of joints. To date, there are no radical curative treatments for OA and novel therapies are mandatory. Recent evidence suggests the role of miRNAs in OA progression. In our previous studies, we demonstrated the role of miR-31-5p and miR-33a families in different bone regeneration signaling. Here, we investigated the role of miR-31-5p and miR-33a-5p in OA progression. A different expression of miR-31-5p and miR-33a-5p into osteoblasts and chondrocytes isolated from joint tissues of OA patients classified in based on different Kellgren and Lawrence (KL) grading was highlighted; and through a bioinformatic approach the common miRNAs target Specificity proteins (Sp1) were identified. Sp1 regulates the expression of gap junction protein Connexin43 (Cx43), which in OA drives the modification of (i) osteoblasts and chondrocytes genes expression, (ii) joint inflammation cytokines releases and (iii) cell functions. Concerning this, thanks to gain and loss of function studies, the possible role of Sp1 as a modulator of CX43 expression through miR-31-5p and miR-33a-5p action was also evaluated. Finally, we hypothesize that both miRNAs cooperate to modulate the expression of SP1 in osteoblasts and chondrocytes and interfering, consequently, with CX43 expression, and they might be further investigated as new possible biomarkers for OA. View Full-Text
Keywords: osteoarthritis; microRNAs; osteoblasts; chondrocytes; SP1; CX43 osteoarthritis; microRNAs; osteoblasts; chondrocytes; SP1; CX43
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MDPI and ACS Style

Costa, V.; De Fine, M.; Carina, V.; Conigliaro, A.; Raimondi, L.; De Luca, A.; Bellavia, D.; Salamanna, F.; Alessandro, R.; Pignatti, G.; Fini, M.; Giavaresi, G. How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights. Int. J. Mol. Sci. 2021, 22, 2471. https://doi.org/10.3390/ijms22052471

AMA Style

Costa V, De Fine M, Carina V, Conigliaro A, Raimondi L, De Luca A, Bellavia D, Salamanna F, Alessandro R, Pignatti G, Fini M, Giavaresi G. How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights. International Journal of Molecular Sciences. 2021; 22(5):2471. https://doi.org/10.3390/ijms22052471

Chicago/Turabian Style

Costa, Viviana, Marcello De Fine, Valeria Carina, Alice Conigliaro, Lavinia Raimondi, Angela De Luca, Daniele Bellavia, Francesca Salamanna, Riccardo Alessandro, Giovanni Pignatti, Milena Fini, and Gianluca Giavaresi. 2021. "How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights" International Journal of Molecular Sciences 22, no. 5: 2471. https://doi.org/10.3390/ijms22052471

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