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Article

ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets

1
Curso de Medicina Veterinária, Universidade Federal do Pampa, Unipampa, Uruguaiana 96460-000, RS, Brazil
2
Departamento de Genética e Evolução, Universidade Federal de São Carlos, UFSCar, São Carlos 13565-905, SP, Brazil
3
Instituto de Química, Universidade Federal de Catalão, UFG/UFCAT, Catalão 75705-220, GO, Brazil
4
Instituto de Química, Universidade de Campinas, UNICAMP, Campinas 13083-970, SP, Brazil
5
Unidade Integrada de Farmacologia e Gastroenterologia, Bragança Paulista 12916-900, SP, Brazil
6
Núcleo de Pesquisa do Mackenzie em Ciência, Fé e Sociedade, Universidade Presbiteriana Mackenzie, São Paulo 01302-907, SP, Brazil
7
Centro de Ciências Naturais e Humana, Universidade Federal do ABC, Santo André 09210-580, SP, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(3), 1311; https://doi.org/10.3390/ijms22031311
Received: 1 October 2020 / Revised: 26 November 2020 / Accepted: 16 December 2020 / Published: 28 January 2021
Embryonic lipids are crucial for the formation of cellular membranes and dynamically participate in metabolic pathways. Cells can synthesize simple fatty acids, and the elongation of fatty acids facilitates the formation of complex lipids. The aim of this work was to investigate the involvement of the elongation of very long chain fatty acid enzyme 5 (ELOVL5) in embryonic development and lipid determination. Bovine embryos were produced in vitro using a standard protocol and randomly divided to receive one of three treatments at Day 4: morpholino (Mo) gene expression knockdown assay for ELOVL5 (ELOVL5-Mo), Mo antisense oligonucleotides for the thalassemic β-globulin human mRNA (technical control Mo), and placebo (biological control). The phenotypes of embryonic development, cell number, ELOVL5 protein abundance, lipid droplet deposits, and lipid fingerprint were investigated. No detrimental effects (p > 0.05) were observed on embryo development in terms of cleavage (59.4 ± 3.5%, 63.6 ± 4.1%, and 65.4 ± 2.2%), blastocyst production (31.3 ± 4.2%, 28.1 ± 4.9%, and 36.1 ± 2.1%), and blastocyst cell number (99.6 ± 7.7, 100.2 ± 6.2, 86.8 ± 5.6), respectively, for biological control, technical control Mo, and ELOVL5-Mo. ELOVL5 protein abundance and cytoplasmic lipid droplet deposition were increased (p < 0.05) in ELOVL5-Mo–derived blastocysts compared with the controls. However, seven lipid species, including phosphatidylcholines, phosphatidylethanolamines, and triacylglycerol, were downregulated in the ELOVL5-Mo–derived blastocysts compared with the biological control. Therefore, ELOVL5 is involved in the determination of embryonic lipid content and composition. Transient translational blockage of ELOVL5 reduced the expression of specific lipid species and promoted increased cytoplasmic lipid droplet deposition, but with no apparent deleterious effect on embryonic development and blastocyst cell number. View Full-Text
Keywords: blastocyst; cytoplasmic lipid deposit; lipid fingerprint; bovine; fatty acid elongation; early embryo development blastocyst; cytoplasmic lipid deposit; lipid fingerprint; bovine; fatty acid elongation; early embryo development
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MDPI and ACS Style

Lanzarini, F.; Pereira, F.A.; Camargo, J.d.; Oliveira, A.M.; Belaz, K.R.A.; Melendez-Perez, J.J.; Eberlin, M.N.; Brum, M.C.S.; Mesquita, F.S.; Sudano, M.J. ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets. Int. J. Mol. Sci. 2021, 22, 1311. https://doi.org/10.3390/ijms22031311

AMA Style

Lanzarini F, Pereira FA, Camargo Jd, Oliveira AM, Belaz KRA, Melendez-Perez JJ, Eberlin MN, Brum MCS, Mesquita FS, Sudano MJ. ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets. International Journal of Molecular Sciences. 2021; 22(3):1311. https://doi.org/10.3390/ijms22031311

Chicago/Turabian Style

Lanzarini, Franciele, Fernanda A. Pereira, Janine d. Camargo, Andressa M. Oliveira, Katia R.A. Belaz, Jose J. Melendez-Perez, Marcos N. Eberlin, Mário C.S. Brum, Fernando S. Mesquita, and Mateus J. Sudano. 2021. "ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets" International Journal of Molecular Sciences 22, no. 3: 1311. https://doi.org/10.3390/ijms22031311

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