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Article

Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice

1
Department of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, Croatia
2
Department of Medical Genetics, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
3
Department of Anatomy, Shiga University of Medical Science, Ötsu 520-2192, Japan
4
Division of Anatomy, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, 981-Miyagi 8558, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Mathieu Vinken
Int. J. Mol. Sci. 2021, 22(3), 1284; https://doi.org/10.3390/ijms22031284
Received: 23 December 2020 / Revised: 24 January 2021 / Accepted: 25 January 2021 / Published: 28 January 2021
(This article belongs to the Special Issue Connexin and Pannexin Signaling in Health and Disease)
Numerous evidence corroborates roles of gap junctions/hemichannels in proper kidney development. We analyzed how Dab1 gene functional silencing influences expression and localization of Cx37, Cx40, Cx43, Cx45, Panx1 and renin in postnatal kidneys of yotari mice, by using immunohistochemistry and electron microscopy. Dab1 Δ102/221 might lead to the activation of c-Src tyrosine kinase, causing the upregulation of Cx43 in the medulla of yotari mice. The expression of renin was more prominent in yotari mice (p < 0.001). Renin granules were unusually present inside the vascular walls of glomeruli capillaries, in proximal and distal convoluted tubules and in the medulla. Disfunction of Cx40 is likely responsible for increased atypically positioned renin cells which release renin in an uncontrolled fashion, but this doesn’t rule out simultaneous involvement of other Cxs, such as Cx45 which was significantly increased in the yotari cortex. The decreased Cx37 expression in yotari medulla might contribute to hypertension reduction provoked by high renin expression. These findings imply the relevance of Cxs/Panx1 as markers of impaired kidney function (high renin) in yotari mice and that they have a role in the preservation of intercellular signaling and implicate connexopathies as the cause of premature death of yotari mice. View Full-Text
Keywords: Cx37; Cx40; Cx43; Cx45; Pnx1; renin; yotari; kidney Cx37; Cx40; Cx43; Cx45; Pnx1; renin; yotari; kidney
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MDPI and ACS Style

Lozić, M.; Filipović, N.; Jurić, M.; Kosović, I.; Benzon, B.; Šolić, I.; Kelam, N.; Racetin, A.; Watanabe, K.; Katsuyama, Y.; Ogata, M.; Saraga-Babić, M.; Vukojević, K. Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice. Int. J. Mol. Sci. 2021, 22, 1284. https://doi.org/10.3390/ijms22031284

AMA Style

Lozić M, Filipović N, Jurić M, Kosović I, Benzon B, Šolić I, Kelam N, Racetin A, Watanabe K, Katsuyama Y, Ogata M, Saraga-Babić M, Vukojević K. Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice. International Journal of Molecular Sciences. 2021; 22(3):1284. https://doi.org/10.3390/ijms22031284

Chicago/Turabian Style

Lozić, Mirela, Natalija Filipović, Marija Jurić, Ivona Kosović, Benjamin Benzon, Ivana Šolić, Nela Kelam, Anita Racetin, Koichiro Watanabe, Yu Katsuyama, Masaki Ogata, Mirna Saraga-Babić, and Katarina Vukojević. 2021. "Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice" International Journal of Molecular Sciences 22, no. 3: 1284. https://doi.org/10.3390/ijms22031284

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