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Article

Identification of the Genetic Basis of Response to de-Acclimation in Winter Barley

1
Department of Plant Breeding, Physiology and Seed Science, University of Agriculture in Kraków, Podłużna 3, 30-239 Krakow, Poland
2
Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland
3
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(3), 1057; https://doi.org/10.3390/ijms22031057
Received: 23 December 2020 / Revised: 15 January 2021 / Accepted: 19 January 2021 / Published: 21 January 2021
(This article belongs to the Special Issue Mechanisms of Freezing Tolerance in Plants)
Mechanisms involved in the de-acclimation of herbaceous plants caused by warm periods during winter are poorly understood. This study identifies the genes associated with this mechanism in winter barley. Seedlings of eight accessions (four tolerant and four susceptible to de-acclimation cultivars and advanced breeding lines) were cold acclimated for three weeks and de-acclimated at 12 °C/5 °C (day/night) for one week. We performed differential expression analysis using RNA sequencing. In addition, reverse-transcription quantitative real-time PCR and enzyme activity analyses were used to investigate changes in the expression of selected genes. The number of transcripts with accumulation level changed in opposite directions during acclimation and de-acclimation was much lower than the number of transcripts with level changed exclusively during one of these processes. The de-acclimation-susceptible accessions showed changes in the expression of a higher number of functionally diverse genes during de-acclimation. Transcripts associated with stress response, especially oxidoreductases, were the most abundant in this group. The results provide novel evidence for the distinct molecular regulation of cold acclimation and de-acclimation. Upregulation of genes controlling developmental changes, typical for spring de-acclimation, was not observed during mid-winter de-acclimation. Mid-winter de-acclimation seems to be perceived as an opportunity to regenerate after stress. Unfortunately, it is competitive to remain in the cold-acclimated state. This study shows that the response to mid-winter de-acclimation is far more expansive in de-acclimation-susceptible cultivars, suggesting that a reduced response to the rising temperature is crucial for de-acclimation tolerance. View Full-Text
Keywords: de-acclimation; freezing tolerance; barley; climate change; RNAseq; gene expression; oxidoreductase de-acclimation; freezing tolerance; barley; climate change; RNAseq; gene expression; oxidoreductase
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MDPI and ACS Style

Wójcik-Jagła, M.; Daszkowska-Golec, A.; Fiust, A.; Kopeć, P.; Rapacz, M. Identification of the Genetic Basis of Response to de-Acclimation in Winter Barley. Int. J. Mol. Sci. 2021, 22, 1057. https://doi.org/10.3390/ijms22031057

AMA Style

Wójcik-Jagła M, Daszkowska-Golec A, Fiust A, Kopeć P, Rapacz M. Identification of the Genetic Basis of Response to de-Acclimation in Winter Barley. International Journal of Molecular Sciences. 2021; 22(3):1057. https://doi.org/10.3390/ijms22031057

Chicago/Turabian Style

Wójcik-Jagła, Magdalena, Agata Daszkowska-Golec, Anna Fiust, Przemysław Kopeć, and Marcin Rapacz. 2021. "Identification of the Genetic Basis of Response to de-Acclimation in Winter Barley" International Journal of Molecular Sciences 22, no. 3: 1057. https://doi.org/10.3390/ijms22031057

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