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Article

Preparative Biocatalytic Synthesis of α-Ketoglutaramate

1
Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University, Lenin Hills 1, Bldg. 40, 119991 Moscow, Russia
2
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Lenin Hills 1, Bldg. 73, 119991 Moscow, Russia
3
Centre for Strategic Planning of FMBA of the Russian Federation, Pogodinskaya St., Bld.10, 119121 Moscow, Russia
*
Authors to whom correspondence should be addressed.
Academic Editor: Walter Wahli
Int. J. Mol. Sci. 2021, 22(23), 12748; https://doi.org/10.3390/ijms222312748
Received: 11 October 2021 / Revised: 15 November 2021 / Accepted: 23 November 2021 / Published: 25 November 2021
(This article belongs to the Section Biochemistry)
α-Ketoglutaramate (KGM) is an underexamined metabolite of L-glutamine in the metabolic pathway of glutaminase II of α-ketoglutarate formation. Presumably, KGM may be a biomarker of hepatic encephalopathy and other hyperammonemic diseases. This metabolite is a substrate for the ω-amidase enzyme and is used to determine its activity in the study of the biochemistry of various types of cancer. However, the commercial unavailability of KGM hinders its widespread use. Methods for the preparative synthesis of KGM are known, but they either do not provide the proper yield or proper purity of the target product. In this work, a detailed description of the procedures is given that allows the production of KGM with a purity above 97% and a yield of the target product above 75% using L-amino acid oxidase from C. adamanteus as a catalyst of L-glutamine conversion. KGM can be obtained both in the form of a highly concentrated aqueous solution and in the form of crystals of sodium salt. The developed methods can be used both for scaling up the synthesis of KGM and for creating economical biocatalytic technologies for the production of other highly purified preparations. View Full-Text
Keywords: α-ketoglutaramate; L-amino acid oxidase; preparative biocatalytic synthesis; ω-amidase; substrate α-ketoglutaramate; L-amino acid oxidase; preparative biocatalytic synthesis; ω-amidase; substrate
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MDPI and ACS Style

Nikulin, M.; Drobot, V.; Švedas, V.; Krasnikov, B.F. Preparative Biocatalytic Synthesis of α-Ketoglutaramate. Int. J. Mol. Sci. 2021, 22, 12748. https://doi.org/10.3390/ijms222312748

AMA Style

Nikulin M, Drobot V, Švedas V, Krasnikov BF. Preparative Biocatalytic Synthesis of α-Ketoglutaramate. International Journal of Molecular Sciences. 2021; 22(23):12748. https://doi.org/10.3390/ijms222312748

Chicago/Turabian Style

Nikulin, Maksim, Viktor Drobot, Vytas Švedas, and Boris F. Krasnikov 2021. "Preparative Biocatalytic Synthesis of α-Ketoglutaramate" International Journal of Molecular Sciences 22, no. 23: 12748. https://doi.org/10.3390/ijms222312748

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