Mutation in OsFWL7 Affects Cadmium and Micronutrient Metal Accumulation in Rice
Abstract
:1. Introduction
2. Results
2.1. Characterization of OsFWL7
2.2. osfwl7 Mutants Are Less Sensitive to Cd
2.3. OsFWL7 Is Involved in Cd Uptake and Translocation
2.4. osfwl7 Mutation Affects Micronutrient Metal Accumulation in Rice
2.5. osfwl7 Mutation Affects the Expression of Heavy Metal Transporter Genes
2.6. Rice FWL Proteins Interact with Themselves and One Another
2.7. Rice FWL Proteins Interact with Membrane Microdomain Marker Proteins
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Subcellular Localization Analysis
4.3. RNA Isolation and RT-qPCR
4.4. Yeast Two-Hybrid Assay
4.5. BiFC Assay
4.6. Measurement of Heavy Metal Levels
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gao, Q.; Liu, L.; Zhou, H.; Liu, X.; Li, W.; Min, Y.; Yan, Y.; Ji, J.; Zhang, H.; Zhao, X. Mutation in OsFWL7 Affects Cadmium and Micronutrient Metal Accumulation in Rice. Int. J. Mol. Sci. 2021, 22, 12583. https://doi.org/10.3390/ijms222212583
Gao Q, Liu L, Zhou H, Liu X, Li W, Min Y, Yan Y, Ji J, Zhang H, Zhao X. Mutation in OsFWL7 Affects Cadmium and Micronutrient Metal Accumulation in Rice. International Journal of Molecular Sciences. 2021; 22(22):12583. https://doi.org/10.3390/ijms222212583
Chicago/Turabian StyleGao, Qingsong, Lei Liu, Haiying Zhou, Xi Liu, Wei Li, Yu Min, Yurong Yan, Jianhui Ji, Hao Zhang, and Xiangxiang Zhao. 2021. "Mutation in OsFWL7 Affects Cadmium and Micronutrient Metal Accumulation in Rice" International Journal of Molecular Sciences 22, no. 22: 12583. https://doi.org/10.3390/ijms222212583