GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy
Abstract
:1. Introduction
2. Results
2.1. Characterization of the Glycosphingolipid Profile in Rat Brain Tissue
2.2. Glycosphingolipid Screening Revealed Changes in Ganglioside and Sulfatide Levels in Aging
2.3. Glycosphingolipid Screening in an SHR24 Rat Model for Tauopathy
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Chemicals and Reagents
4.3. Sample Preparation
4.4. UHPLC-Q-TOF Analysis
4.5. Immunohistochemical Staining
4.6. Preparation of Sarkosyl-Insoluble PHF-tau from Transgenic Rat Brains
4.7. Biochemical Western Blot Analysis
4.8. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Olešová, D.; Majerová, P.; Hájek, R.; Piešťanský, J.; Brumarová, R.; Michalicová, A.; Jurkanin, B.; Friedecký, D.; Kováč, A. GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy. Int. J. Mol. Sci. 2021, 22, 12581. https://doi.org/10.3390/ijms222212581
Olešová D, Majerová P, Hájek R, Piešťanský J, Brumarová R, Michalicová A, Jurkanin B, Friedecký D, Kováč A. GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy. International Journal of Molecular Sciences. 2021; 22(22):12581. https://doi.org/10.3390/ijms222212581
Chicago/Turabian StyleOlešová, Dominika, Petra Majerová, Roman Hájek, Juraj Piešťanský, Radana Brumarová, Alena Michalicová, Bernadeta Jurkanin, David Friedecký, and Andrej Kováč. 2021. "GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy" International Journal of Molecular Sciences 22, no. 22: 12581. https://doi.org/10.3390/ijms222212581