Mediation of the Cardioprotective Effects of Mannitol Discovered, with Refutation of Common Protein Kinases
Abstract
:1. Introduction
2. Results
2.1. Animal Characteristics
2.2. Infarct Size
2.3. Cardiac Function
3. Discussion
Limitations
4. Materials and Methods
4.1. Surgical Preparation
4.2. Experimental Protocol
4.3. Statistical Analysis
4.3.1. Sample Size Analysis
4.3.2. Statistical Approach
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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n | Body Weight (g) | Heart Weight Dry (g) | Heart Weight Wet (g) | Time of Max. Ischemic Contracture (min) | Level of Max. Ischemic Contracture (mmHg) | |
---|---|---|---|---|---|---|
Con | 7 | 304 ± 29 | 0.17 ± 0.02 | 1.13 ± 0.08 | 15 ± 1 | 66 ± 14 |
Man | 7 | 313 ± 28 | 0.17 ± 0.02 | 1.10 ± 0.08 | 16 ± 2 | 56 ± 10 |
Man + MK | 7 | 308 ± 12 | 0.16 ± 0.01 | 1.07 ± 0.04 | 15 ± 1 | 57 ± 8 |
Man + KT | 7 | 312 ± 10 | 0.17 ± 0.01 | 1.08 ± 0.05 | 16 ± 2 | 60 ± 9 |
Man + DPCPX | 7 | 278 ± 10 | 0.15 ± 0.01 | 1.05 ± 0.04 | 14 ± 2 | 65 ± 19 |
DPCPX | 7 | 290 ± 17 * | 0.16 ± 0.01 | 1.09 ± 0.04 | 15 ± 2 | 71 ± 10 |
Baseline | PC | Reperfusion | ||
---|---|---|---|---|
30 | 60 | |||
Heart Rate (bpm) | ||||
Con | 306 ± 26 | 281 ± 37 | 273 ± 43 | 255 ± 41 |
Man | 296 ± 27 | 297 ± 22 | 278 ± 62 | 263 ± 21 |
Man + MK | 306 ± 24 | 300 ± 34 | 308 ± 58 | 293 ± 69 |
Man + KT | 318 ± 28 | 313 ± 28 | 273 ± 43 | 287 ± 33 |
Man + DPCPX | 305 ± 37 | 311 ± 36 | 285 ± 68 | 248 ± 52 |
DPCPX | 290 ± 34 | 298 ± 30 | 290 ± 18 | 246 ± 61 |
Left Ventricular Developed Pressure (mmHg) | ||||
Con | 139 ± 16 | 146 ± 14 | 22 ± 15 * | 28 ± 10 * |
Man | 137 ± 16 | 135 ± 18 | 25 ± 6 * | 34 ± 4 * |
Man + MK | 143 ± 16 | 141 ± 18 | 29 ± 14 * | 34 ± 19 * |
Man + KT | 134 ± 6 | 137 ± 6 | 20 ± 10 * | 31 ± 11 * |
Man + DPCPX | 141 ± 23 | 136 ± 23 | 18 ± 16 * | 31 ± 11 * |
DPCPX | 136 ± 18 | 136 ± 17 | 23 ± 16 * | 33 ± 17 * |
Coronary flow (mL/min) | ||||
Con | 15 ± 2 | 15 ± 1 | 8 ± 1 * | 7 ± 1 * |
Man | 18 ± 3 | 18 ± 4 | 10 ± 4 * | 9 ± 3 * |
Man + MK | 16 ± 2 | 16 ± 2 | 10 ± 3 * | 9 ± 2 * |
Man + KT | 14 ± 2 | 15 ± 1 | 8 ± 2 * | 7 ± 2 * |
Man + DPCPX | 15 ± 1 | 15 ± 1 | 8 ± 1 * | 8 ± 1 * |
DPCPX | 15 ± 3 | 15 ± 3 | 8 ± 3 * | 7 ± 3 * |
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Torregroza, C.; Glashoerster, C.O.; Feige, K.; Stroethoff, M.; Raupach, A.; Heinen, A.; Hollmann, M.W.; Huhn, R. Mediation of the Cardioprotective Effects of Mannitol Discovered, with Refutation of Common Protein Kinases. Int. J. Mol. Sci. 2021, 22, 12471. https://doi.org/10.3390/ijms222212471
Torregroza C, Glashoerster CO, Feige K, Stroethoff M, Raupach A, Heinen A, Hollmann MW, Huhn R. Mediation of the Cardioprotective Effects of Mannitol Discovered, with Refutation of Common Protein Kinases. International Journal of Molecular Sciences. 2021; 22(22):12471. https://doi.org/10.3390/ijms222212471
Chicago/Turabian StyleTorregroza, Carolin, Chiara O. Glashoerster, Katharina Feige, Martin Stroethoff, Annika Raupach, André Heinen, Markus W. Hollmann, and Ragnar Huhn. 2021. "Mediation of the Cardioprotective Effects of Mannitol Discovered, with Refutation of Common Protein Kinases" International Journal of Molecular Sciences 22, no. 22: 12471. https://doi.org/10.3390/ijms222212471