Chymase as a Novel Therapeutic Target in Acute Pancreatitis
Abstract
:1. Introduction
2. Results
2.1. Two Doses of L-Arginine-Induced Acute Pancreatitis Models
2.2. Time Course of the High-Dose L-Arginine-Induced Model of Acute Pancreatitis
2.3. Effects of Chymase Inhibitor on Serum Lipase and Pancreatic TNF-α mRNA Levels
2.4. Chymase Inhibitor TY-51469 Decreases Immune Cell Infiltration into the Pancreas
2.5. Chymase Inhibitor TY-51469 Suppressed Pancreatic Chymase and MMP-9 Activities
2.6. Chymase Inhibitor TY-51469 Decreases the Number of Chymase-Positive Cells in the Pancreas
2.7. Chymase Inhibitor TY-51469 Increases the Survival Rate of Hamsters
3. Discussion
4. Material and Methods
4.1. Drug
4.2. Animals and Experimental Design
4.3. Serum Lipase
4.4. Preparation of the Pancreas
4.5. Histological Analysis
4.6. Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)
4.7. Enzyme Activities
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kuramoto, T.; Jin, D.; Komeda, K.; Taniguchi, K.; Hirokawa, F.; Takai, S.; Uchiyama, K. Chymase as a Novel Therapeutic Target in Acute Pancreatitis. Int. J. Mol. Sci. 2021, 22, 12313. https://doi.org/10.3390/ijms222212313
Kuramoto T, Jin D, Komeda K, Taniguchi K, Hirokawa F, Takai S, Uchiyama K. Chymase as a Novel Therapeutic Target in Acute Pancreatitis. International Journal of Molecular Sciences. 2021; 22(22):12313. https://doi.org/10.3390/ijms222212313
Chicago/Turabian StyleKuramoto, Toru, Denan Jin, Koji Komeda, Kohei Taniguchi, Fumitoshi Hirokawa, Shinji Takai, and Kazuhisa Uchiyama. 2021. "Chymase as a Novel Therapeutic Target in Acute Pancreatitis" International Journal of Molecular Sciences 22, no. 22: 12313. https://doi.org/10.3390/ijms222212313