Aging, Bone Marrow and Next-Generation Sequencing (NGS): Recent Advances and Future Perspectives
Abstract
:1. Introduction
2. Aging in the Bone Marrow
2.1. The Aging Bone Marrow (BM) Niche
2.2. Age-Related Changes in Haematopoietic Stem Cells (HSCs)
2.3. Age-Related Changes in Mesenchymal Stem Cells (MSCs)
2.4. Age-Related Changes in B Cells and T Cells: Immune Aging and Immunosenescence
2.4.1. B Cells
2.4.2. T Cells
3. NGS and Its Applications in Aging Studies
3.1. Targeted Sequencing Approaches
3.1.1. DNA Panels
3.1.2. ScRNA and Bulk RNA-Seq
4. The Future of NGS in Aging Studies—Potential, Perspectives and Limitations
4.1. Potential and Perspectives of NGS in Aging Studies
4.2. Limitations of NGS in Aging Studies
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Cells Explored | DNA/RNA | Key Findings | Reference |
---|---|---|---|
SSCs | RNA |
| [82] |
Non-HSCs (MSCs) | RNA |
| [83] |
MSC lineage cells | RNA |
| [84] |
HSCs and MSCs | RNA |
| [71] |
BM cells with focus on T cells | RNA |
| [73] |
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Ganguly, P.; Toghill, B.; Pathak, S. Aging, Bone Marrow and Next-Generation Sequencing (NGS): Recent Advances and Future Perspectives. Int. J. Mol. Sci. 2021, 22, 12225. https://doi.org/10.3390/ijms222212225
Ganguly P, Toghill B, Pathak S. Aging, Bone Marrow and Next-Generation Sequencing (NGS): Recent Advances and Future Perspectives. International Journal of Molecular Sciences. 2021; 22(22):12225. https://doi.org/10.3390/ijms222212225
Chicago/Turabian StyleGanguly, Payal, Bradley Toghill, and Shelly Pathak. 2021. "Aging, Bone Marrow and Next-Generation Sequencing (NGS): Recent Advances and Future Perspectives" International Journal of Molecular Sciences 22, no. 22: 12225. https://doi.org/10.3390/ijms222212225