Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase
Abstract
:1. Introduction
2. Results
2.1. puc Genomic Domain Analysis
2.2. Analysis of Lines Carrying puc Regions In Vivo
2.3. Upstream Sequences (PG1)
2.4. First and Second Intron (PG2, RS1)
2.5. Third Intron (PG3, PG4, RS2–4)
2.6. Downstream Sequences (PG5)
2.7. Regulatory Control of puc Expression by the JNK Signaling Cascade
3. Discussion
3.1. puc Expression Regulatory Domains
3.2. puc Expression Regulatory Factors
4. Methods
4.1. Cloning of puc Regulatory Sequences
4.2. Bioinformatic Analyses
4.3. Drosophila Strains and Genetics
4.4. Drosophila Embryo Fixation and Dissections
4.5. Immunohistochemistry
4.6. Image Acquisition
4.7. Larvae Live Imaging
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Embryo | CNS Embryo/1st Instar Larvae | 3rd Instar Larvae | |
---|---|---|---|
PG1 | Salivary Glands | Salivary Glands | |
Posterior Spiracles | Posterior Spiracles | ||
Brain lobes Neurons | Denticles precursors | ||
Thoracic ganglion Neurons | |||
PG2 | Posterior Spiracles | Posterior Spiracles | |
CNS | CNS Glia | CNS | |
Epidermis (patchy) | · Perineural/Subperineural | · VNC subset | |
Tracheal System | · Ensheating | · Glia intersegmental nerve | |
Foregut | Epidermis | ||
Tracheal System | |||
Foregut | |||
Posterior midgut | |||
Anal pad | |||
RS1 | Salivary Glands | ||
Posterior Spiracles | Posterior Spiracles | ||
Hindgut | Epidermis (patchy) | ||
Maxillary primordia | Tracheal System (patchy) | ||
Epidermis | Maxillary primordia | ||
PG3 | Salivary Glands | Salivary Glands | |
CNS | CNS Glia | Midgut/Hindgut | |
Midgut (weak) | · Repo-negative midline | Body wall muscles | |
Body wall muscles | · Surface | Heart | |
Pharynx/ring gland | VNC tracheal arborization | Alary muscles | |
RS2 | Salivary Glands | Salivary Glands | |
Posterior Spiracles | |||
CNS | CNS | Body wall muscles (subset) | |
Body wall muscles (subset) | · Neurons | Heart | |
Healing Epithelia | · Glia | Alary muscles | |
Midgut | Anal pad periphery | ||
RS3 | Salivary Glands | Salivary Glands | |
Posterior Spiracles | Posterior Spiracles | ||
Epidermis | Epidermis | ||
Tracheal System | Tracheal System | ||
· Dorsal trunks | |||
· Distal arborization | |||
RS4 | Salivary Glands | Salivary Glands | |
Posterior Spiracles | Posterior Spiracles | ||
Tracheal System | |||
Anal pad | |||
Maxillary primordia | |||
PG4 | Salivary Glands | Salivary Glands | |
Posterior Spiracles | Posterior Spiracles | ||
Epidermis (patchy) | Brain lobes/VNC Interneurons | Epidermis (patchy) | |
Ring gland | Nerves axonal projections | Ring gland | |
Astrocytes | Posterior midgut | ||
Ensheathing glia | Tracheal junctions | ||
PG5 | Salivary Glands | Salivary Glands | |
Posterior Spiracles | Posterior Spiracles | ||
Epidermis (scattered) | Epidermis (scattered) | ||
Maxillary primordia? |
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Karkali, K.; Martin-Blanco, E. Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase. Int. J. Mol. Sci. 2021, 22, 12205. https://doi.org/10.3390/ijms222212205
Karkali K, Martin-Blanco E. Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase. International Journal of Molecular Sciences. 2021; 22(22):12205. https://doi.org/10.3390/ijms222212205
Chicago/Turabian StyleKarkali, Katerina, and Enrique Martin-Blanco. 2021. "Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase" International Journal of Molecular Sciences 22, no. 22: 12205. https://doi.org/10.3390/ijms222212205