Fukutin Protein Participates in Cell Proliferation by Enhancing Cyclin D1 Expression through Binding to the Transcription Factor Activator Protein-1: An In Vitro Study
Abstract
:1. Introduction
2. Results
2.1. Fukutin Is Localized in Both the Nucleus and Cytoplasm
2.2. Knockdown and Overexpression of Fukutin Alter Proliferation Ability
2.3. Knockdown and Overexpression of Fukutin Alter Cyclin D1 Transcript and Protein Levels
2.4. Fukutin Protein Binds to the Promoter Region of Cyclin D1
2.5. Fukutin Protein Binds to the AP-1 Component c-Jun/p-c-Jun
2.6. JNK Inhibition Downregulates Cyclin D1 Expression
2.7. Knockdown or Overexpression of Fukutin Does Not Affect Phosphorylation of JNK or c-Jun
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Experimental Design
4.2. Knockdown Fukutin
4.3. Overexpression of Fukutin
4.4. JNK Inhibition
4.5. Immunocytochemistry on Cell Block Sections
4.6. Immunocytochemistry on Culture Slides
4.7. Semiquantitative RT-PCR Analysis
4.8. Protein Extraction
4.9. Immunoprecipitation
4.10. Western Blot Analysis
4.11. Preparation of DNA Probes for ELISA-Based Assay
4.12. ELISA
4.13. Sandwich ELISA
4.14. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Okamura, Y.; Yamamoto, T.; Tsukui, R.; Kato, Y.; Shibata, N. Fukutin Protein Participates in Cell Proliferation by Enhancing Cyclin D1 Expression through Binding to the Transcription Factor Activator Protein-1: An In Vitro Study. Int. J. Mol. Sci. 2021, 22, 12153. https://doi.org/10.3390/ijms222212153
Okamura Y, Yamamoto T, Tsukui R, Kato Y, Shibata N. Fukutin Protein Participates in Cell Proliferation by Enhancing Cyclin D1 Expression through Binding to the Transcription Factor Activator Protein-1: An In Vitro Study. International Journal of Molecular Sciences. 2021; 22(22):12153. https://doi.org/10.3390/ijms222212153
Chicago/Turabian StyleOkamura, Yukinori, Tomoko Yamamoto, Ryota Tsukui, Yoichiro Kato, and Noriyuki Shibata. 2021. "Fukutin Protein Participates in Cell Proliferation by Enhancing Cyclin D1 Expression through Binding to the Transcription Factor Activator Protein-1: An In Vitro Study" International Journal of Molecular Sciences 22, no. 22: 12153. https://doi.org/10.3390/ijms222212153