Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin
Abstract
:1. Introduction
2. Results
2.1. Selection of gRNA for DSB-Induction
2.2. Analysis of Targeted IHR Events
2.3. PacBio® Sequencing for Somatic Recombination Analysis
3. Discussion
3.1. IHR Frequency
3.2. Conversion Tract Patterns
3.3. Somatic IHR
3.4. IHR in Euchromatin versus Heterochromatin
4. Materials and Methods
4.1. Plant Material
4.2. Plasmids and Plant Transformation
4.3. DNA Amplification and Sequencing
4.4. Sequence Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Filler-Hayut, S.; Kniazev, K.; Melamed-Bessudo, C.; Levy, A.A. Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin. Int. J. Mol. Sci. 2021, 22, 12096. https://doi.org/10.3390/ijms222212096
Filler-Hayut S, Kniazev K, Melamed-Bessudo C, Levy AA. Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin. International Journal of Molecular Sciences. 2021; 22(22):12096. https://doi.org/10.3390/ijms222212096
Chicago/Turabian StyleFiller-Hayut, Shdema, Kiril Kniazev, Cathy Melamed-Bessudo, and Avraham A. Levy. 2021. "Targeted Inter-Homologs Recombination in Arabidopsis Euchromatin and Heterochromatin" International Journal of Molecular Sciences 22, no. 22: 12096. https://doi.org/10.3390/ijms222212096