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Open AccessArticle

Identification and Functional Analysis of Tomato TPR Gene Family

1
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
2
College of Plant Protection, China Agricultural University, Beijing 100000, China
3
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China
4
College of Sciences, Northeast Agricultural University, Harbin 150030, China
*
Authors to whom correspondence should be addressed.
Co-Author: They contributed equally to this manuscript.
Int. J. Mol. Sci. 2021, 22(2), 758; https://doi.org/10.3390/ijms22020758
Received: 20 November 2020 / Revised: 10 January 2021 / Accepted: 11 January 2021 / Published: 13 January 2021
(This article belongs to the Section Molecular Plant Sciences)
Tomato (Solanum lycopersicum) as an important vegetable grown around the world is threatened by many diseases, which seriously affects its yield. Therefore, studying the interaction between tomato and pathogenic bacteria is biologically and economically important. The TPR (Tetratricopeptide repeat) gene family is a class of genes containing TPR conserved motifs, which are widely involved in cell cycle regulation, gene expression, protein degradation and other biological processes. The functions of TPR gene in Arabidopsis and wheat plants have been well studied, but the research on TPR genes in tomato is not well studied. In this study, 26 TPR gene families were identified using bioinformatics based on tomato genome data, and they were analyzed for subcellular localization, phylogenetic evolution, conserved motifs, tissue expression, and GO (Gene Ontology) analysis. The qRT-PCR was used to detect the expression levels of each member of the tomato TPR gene family (SlTPRs) under biological stress (Botrytis cinerea) and abiotic stress such as drought and abscisic acid (ABA). The results showed that members of the tomato TPR family responded to various abiotic stresses and Botrytis cinerea stress, and the SlTPR2 and SlTPR4 genes changed significantly under different stresses. Using VIGS (Virus-induced gene silencing) technology to silence these two genes, the silenced plants showed reduced disease resistance. It was also shown that TPR4 can interact with atpA which encodes a chloroplast ATP synthase CF1 α subunit. The above results provide a theoretical basis for further exploring the molecular mechanism of TPR-mediated resistance in disease defense, and also provide a foundation for tomato disease resistance breeding. View Full-Text
Keywords: bioinformatics; disease resistance; molecular mechanism; tomato; TPR gene family; SlTPR4 bioinformatics; disease resistance; molecular mechanism; tomato; TPR gene family; SlTPR4
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MDPI and ACS Style

Zhou, X.; Zheng, Y.; Cai, Z.; Wang, X.; Liu, Y.; Yu, A.; Chen, X.; Liu, J.; Zhang, Y.; Wang, A. Identification and Functional Analysis of Tomato TPR Gene Family. Int. J. Mol. Sci. 2021, 22, 758. https://doi.org/10.3390/ijms22020758

AMA Style

Zhou X, Zheng Y, Cai Z, Wang X, Liu Y, Yu A, Chen X, Liu J, Zhang Y, Wang A. Identification and Functional Analysis of Tomato TPR Gene Family. International Journal of Molecular Sciences. 2021; 22(2):758. https://doi.org/10.3390/ijms22020758

Chicago/Turabian Style

Zhou, Xi’nan; Zheng, Yangyang; Cai, Zhibo; Wang, Xingyuan; Liu, Yang; Yu, Anzhou; Chen, Xiuling; Liu, Jiayin; Zhang, Yao; Wang, Aoxue. 2021. "Identification and Functional Analysis of Tomato TPR Gene Family" Int. J. Mol. Sci. 22, no. 2: 758. https://doi.org/10.3390/ijms22020758

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