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Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases

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Division of Dermatological Allergology, Department of Dermatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary
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Department of Dermatology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary
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Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, University of Debrecen, 4032 Debrecen, Hungary
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Department of Pathology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary
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Department of Forensic Medicine, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary
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Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary
*
Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Academic Editor: Lucie Germain
Int. J. Mol. Sci. 2021, 22(19), 10428; https://doi.org/10.3390/ijms221910428
Received: 13 August 2021 / Revised: 15 September 2021 / Accepted: 23 September 2021 / Published: 27 September 2021
(This article belongs to the Section Biochemistry)
The chemical milieu, microbiota composition, and immune activity show prominent differences in distinct healthy skin areas. The objective of the current study was to compare the major permeability barrier components (stratum corneum and tight junction (TJ)), investigate the distribution of (corneo)desmosomes and TJs, and measure barrier function in healthy sebaceous gland-rich (SGR), apocrine gland-rich (AGR), and gland-poor (GP) skin regions. Molecules involved in cornified envelope (CE) formation, desquamation, and (corneo)desmosome and TJ organization were investigated at the mRNA and protein levels using qRT-PCR and immunohistochemistry. The distribution of junction structures was visualized using confocal microscopy. Transepidermal water loss (TEWL) functional measurements were also performed. CE intracellular structural components were similarly expressed in gland-rich (SGR and AGR) and GP areas. In contrast, significantly lower extracellular protein levels of (corneo)desmosomes (DSG1 and CDSN) and TJs (OCLN and CLDN1) were detected in SGR/AGR areas compared to GP areas. In parallel, kallikrein proteases were significantly higher in gland-rich regions. Moreover, gland-rich areas were characterized by prominently disorganized junction structures ((corneo)desmosomes and TJs) and significantly higher TEWL levels compared to GP skin, which exhibited a regular distribution of junction structures. According to our findings, the permeability barrier of our skin is not uniform. Gland-rich areas are characterized by weaker permeability barrier features compared with GP regions. These findings have important clinical relevance and may explain the preferred localization of acantholytic skin diseases on gland-rich skin regions (e.g., Pemphigus foliaceus, Darier’s disease, and Hailey–Hailey disease). View Full-Text
Keywords: acantolytic diseases; barrier function; cornified envelope; Darier’s disease; desmosome; Hailey–Hailey disease; keratinocyte; tight junction acantolytic diseases; barrier function; cornified envelope; Darier’s disease; desmosome; Hailey–Hailey disease; keratinocyte; tight junction
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MDPI and ACS Style

Kapitány, A.; Medgyesi, B.; Jenei, A.; Somogyi, O.; Szabó, L.; Gáspár, K.; Méhes, G.; Hendrik, Z.; Dócs, K.; Szücs, P.; Dajnoki, Z.; Szegedi, A. Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases. Int. J. Mol. Sci. 2021, 22, 10428. https://doi.org/10.3390/ijms221910428

AMA Style

Kapitány A, Medgyesi B, Jenei A, Somogyi O, Szabó L, Gáspár K, Méhes G, Hendrik Z, Dócs K, Szücs P, Dajnoki Z, Szegedi A. Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases. International Journal of Molecular Sciences. 2021; 22(19):10428. https://doi.org/10.3390/ijms221910428

Chicago/Turabian Style

Kapitány, Anikó, Barbara Medgyesi, Adrienn Jenei, Orsolya Somogyi, Lilla Szabó, Krisztián Gáspár, Gábor Méhes, Zoltán Hendrik, Klaudia Dócs, Péter Szücs, Zsolt Dajnoki, and Andrea Szegedi. 2021. "Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases" International Journal of Molecular Sciences 22, no. 19: 10428. https://doi.org/10.3390/ijms221910428

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