Comparative Transcriptome Analysis of Chemoreception Organs of Laodelphax striatellus in Response to Rice Stripe Virus Infection
Abstract
:1. Introduction
2. Results
2.1. Illumina Sequencing and Assembly of the Chemoreception Organs of Female and Male Laodelphax striatellus
2.2. Gene Ontology Classification of Differentially Expressed Genes among the Chemoreception Organs of Females and Males
2.3. Differentially Expressed Genes Induced by RSV Infection
2.4. Gene Ontology Classification of Differentially Expressed Genes Induced by RSV Infection
2.5. Changes in the Expression of Olfactory-Related Genes in the Chemoreception Organs Infected by RSV
2.6. Validation of the OBPs Expressions in RSV-Infected Chemoreception Organs by Quantitative Real-Time PCR
3. Discussion
4. Materials and Methods
4.1. Nonviruliferous and Viruliferous L. striatellus Rearing
4.2. Samples Preparation and Transcriptomic Sequencing
4.3. Transcriptomic Assembly and GO Annotation
4.4. Analysis of Differentially Expressed Genes
4.5. Identification and Comparative Expression Profiles of Olfactory Related Genes
4.6. Quantitative Real-Time PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
DEGs | Differentially expressed genes |
RSV | Rice stripe virus |
CMV | Cucumber mosaic virus |
PLV | Potato leafroll virus |
TYLCV | Tomato yellow leaf curl virus |
SRBDV | Southern rice black streaked dwarf virus |
TCV | Tomato chlorosis virus |
OBPs | Odorant-binding proteins |
CSPs | Chemosensory proteins |
IRs | Ionotropic receptors |
GRs | Gustatory receptors |
ORs | Odorant receptors |
ORco | Olfactory co-receptor receptor |
SNMPs | Sensory neuron membrane proteins |
VFA | Viruliferous female antennae |
VMA | Viruliferous male antennae |
VFL | Viruliferous female legs |
VML | Viruliferous male legs |
VFH | Viruliferous female heads |
VMH | Viruliferous male heads |
NFA | Nonviruliferous female antennae |
NMA | Nonviruliferous male antennae |
NFL | Nonviruliferous female legs |
NML | Nonviruliferous male legs |
NFH | Nonviruliferous female heads |
NMH | Nonviruliferous male heads |
FPKM | Fragments per Kilobase of transcript per Million mapped reads |
PCA | Principal component analysis |
GO | Gene ontology |
qPCR | Real-Time Quantitative PCR |
VOC | volatile organic compounds |
GC–EAD | gas chromatography–electroantennographic detection |
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Li, Y.; Zhang, Y.; Xiang, Y.; Chen, D.; Hu, J.; Liu, F. Comparative Transcriptome Analysis of Chemoreception Organs of Laodelphax striatellus in Response to Rice Stripe Virus Infection. Int. J. Mol. Sci. 2021, 22, 10299. https://doi.org/10.3390/ijms221910299
Li Y, Zhang Y, Xiang Y, Chen D, Hu J, Liu F. Comparative Transcriptome Analysis of Chemoreception Organs of Laodelphax striatellus in Response to Rice Stripe Virus Infection. International Journal of Molecular Sciences. 2021; 22(19):10299. https://doi.org/10.3390/ijms221910299
Chicago/Turabian StyleLi, Yao, Yunye Zhang, Yin Xiang, Danyu Chen, Jia Hu, and Fang Liu. 2021. "Comparative Transcriptome Analysis of Chemoreception Organs of Laodelphax striatellus in Response to Rice Stripe Virus Infection" International Journal of Molecular Sciences 22, no. 19: 10299. https://doi.org/10.3390/ijms221910299