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Article

Genome-Wide Identification, Characterization and Expression Analysis of the JAZ Gene Family in Resistance to Gray Leaf Spots in Tomato

School of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China
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Academic Editor: Hikmet Budak
Int. J. Mol. Sci. 2021, 22(18), 9974; https://doi.org/10.3390/ijms22189974
Received: 30 July 2021 / Revised: 7 September 2021 / Accepted: 14 September 2021 / Published: 15 September 2021
(This article belongs to the Section Molecular Plant Sciences)
The plant disease resistance system involves a very complex regulatory network in which jasmonates play a key role in response to external biotic or abiotic stresses. As inhibitors of the jasmonic acid (JA) signaling pathway, JASMONATE ZIM domain (JAZ) proteins have been identified in many plant species, and their functions are gradually being clarified. In this study, 26 JAZ genes were identified in tomato. The physical and chemical properties, predicted subcellular localization, gene structure, cis-acting elements, and interspecies collinearity of 26 SlJAZ genes were subsequently analyzed. RNA-seq data combined with qRT-PCR analysis data showed that the expression of most SlJAZ genes were induced in response to Stemphylium lycopersici, methyl jasmonate (MeJA) and salicylic acid (SA). Tobacco rattle virus RNA2-based VIGS vector (TRV2)-SlJAZ25 plants were more resistant to tomato gray leaf spots than TRV2-00 plants. Therefore, we speculated that SlJAZ25 played a negative regulatory role in tomato resistance to gray leaf spots. Based on combining the results of previous studies and those of our experiments, we speculated that SlJAZ25 might be closely related to JA and SA hormone regulation. SlJAZ25 interacted with SlJAR1, SlCOI1, SlMYC2, and other resistance-related genes to form a regulatory network, and these genes played an important role in the regulation of tomato gray leaf spots. The subcellular localization results showed that the SlJAZ25 gene was located in the nucleus. Overall, this study is the first to identify and analyze JAZ family genes in tomato via bioinformatics approaches, clarifying the regulatory role of SlJAZ25 genes in tomato resistance to gray leaf spots and providing new ideas for improving plant disease resistance. View Full-Text
Keywords: Solanum lycopersicum; JAZ gene; gray leaf spots; functional verification Solanum lycopersicum; JAZ gene; gray leaf spots; functional verification
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MDPI and ACS Style

Sun, Y.; Liu, C.; Liu, Z.; Zhao, T.; Jiang, J.; Li, J.; Xu, X.; Yang, H. Genome-Wide Identification, Characterization and Expression Analysis of the JAZ Gene Family in Resistance to Gray Leaf Spots in Tomato. Int. J. Mol. Sci. 2021, 22, 9974. https://doi.org/10.3390/ijms22189974

AMA Style

Sun Y, Liu C, Liu Z, Zhao T, Jiang J, Li J, Xu X, Yang H. Genome-Wide Identification, Characterization and Expression Analysis of the JAZ Gene Family in Resistance to Gray Leaf Spots in Tomato. International Journal of Molecular Sciences. 2021; 22(18):9974. https://doi.org/10.3390/ijms22189974

Chicago/Turabian Style

Sun, Yaoguang, Chunxin Liu, Zengbing Liu, Tingting Zhao, Jingbin Jiang, Jingfu Li, Xiangyang Xu, and Huanhuan Yang. 2021. "Genome-Wide Identification, Characterization and Expression Analysis of the JAZ Gene Family in Resistance to Gray Leaf Spots in Tomato" International Journal of Molecular Sciences 22, no. 18: 9974. https://doi.org/10.3390/ijms22189974

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