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Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress

Department of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea
Department of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, Korea
Department of Veterinary Toxicology, College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, Korea
New Drug Development Center, Osong Medical Innovation Foundation, Chungbuk 28160, Korea
Department of Orthopedic Surgery, Yeungnam University College of Medicine, Yeungnam University Medical Center, 170 Hyonchung-ro, Namgu, Daegu 42415, Korea
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Marcin Majka
Int. J. Mol. Sci. 2021, 22(18), 9960;
Received: 22 April 2021 / Revised: 21 June 2021 / Accepted: 23 June 2021 / Published: 15 September 2021
(This article belongs to the Special Issue Stem Cells—from Bench to Bedside 2021)
Stress is the physical and psychological tension felt by an individual while adapting to difficult situations. Stress is known to alter the expression of stress hormones and cause neuroinflammation in the brain. In this study, miRNAs in serum-derived neuronal exosomes (nEVs) were analyzed to determine whether differentially expressed miRNAs could be used as biomarkers of acute stress. Specifically, acute severe stress was induced in Sprague-Dawley rats via electric foot-shock treatment. In this acute severe-stress model, time-dependent changes in the expression levels of stress hormones and neuroinflammation-related markers were analyzed. In addition, nEVs were isolated from the serum of control mice and stressed mice at various time points to determine when brain damage was most prominent; this was found to be 7 days after foot shock. Next-generation sequencing was performed to compare neuronal exosomal miRNA at day 7 with the neuronal exosomal miRNA of the control group. From this analysis, 13 upregulated and 11 downregulated miRNAs were detected. These results show that specific miRNAs are differentially expressed in nEVs from an acute severe-stress animal model. Thus, this study provides novel insights into potential stress-related biomarkers. View Full-Text
Keywords: acute severe stress; neuroinflammation; exosomes; miRNA; biomarkers acute severe stress; neuroinflammation; exosomes; miRNA; biomarkers
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MDPI and ACS Style

Sung, M.; Sung, S.-E.; Kang, K.-K.; Choi, J.-H.; Lee, S.; Kim, K.; Lim, J.-H.; Lee, G.W.; Rim, H.-D.; Kim, B.-S.; Won, S.; Kim, K.; Jang, S.; Seo, M.-S.; Woo, J. Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress. Int. J. Mol. Sci. 2021, 22, 9960.

AMA Style

Sung M, Sung S-E, Kang K-K, Choi J-H, Lee S, Kim K, Lim J-H, Lee GW, Rim H-D, Kim B-S, Won S, Kim K, Jang S, Seo M-S, Woo J. Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress. International Journal of Molecular Sciences. 2021; 22(18):9960.

Chicago/Turabian Style

Sung, Minkyoung, Soo-Eun Sung, Kyung-Ku Kang, Joo-Hee Choi, Sijoon Lee, KilSoo Kim, Ju-Hyeon Lim, Gun W. Lee, Hyo-Deog Rim, Byung-Soo Kim, Seunghee Won, Kyungmin Kim, Seoyoung Jang, Min-Soo Seo, and Jungmin Woo. 2021. "Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress" International Journal of Molecular Sciences 22, no. 18: 9960.

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