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Article

Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation

1
Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, Calwerstraße 7/1, 72076 Tuebingen, Germany
2
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Osianderstr. 2–8, 72076 Tübingen, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Silvia Panseri and Monica Montesi
Int. J. Mol. Sci. 2021, 22(18), 9942; https://doi.org/10.3390/ijms22189942
Received: 18 August 2021 / Revised: 8 September 2021 / Accepted: 10 September 2021 / Published: 14 September 2021
(This article belongs to the Special Issue Cell-Biomaterial Interaction 2021)
Tissue engineering offers auspicious opportunities in oral and maxillofacial surgery to heal bone defects. For this purpose, the combination of cells with stability-providing scaffolds is required. Jaw periosteal cells (JPCs) are well suited for regenerative therapies, as they are easily accessible and show strong osteogenic potential. In this study, we analyzed the influence of uncoated and polylactic-co-glycolic acid (PLGA)-coated β-tricalcium phosphate (β-TCP) scaffolds on JPC colonization and subsequent osteogenic differentiation. Furthermore, interaction with the human blood was investigated. This study demonstrated that PLGA-coated and uncoated β-TCP scaffolds can be colonized with JPCs and further differentiated into osteogenic cells. On day 15, after cell seeding, JPCs with and without osteogenic differentiation were incubated with fresh human whole blood under dynamic conditions. The activation of coagulation, complement system, inflammation, and blood cells were analyzed using ELISA and scanning electron microscopy (SEM). JPC-seeded scaffolds showed a dense cell layer and osteogenic differentiation capacity on both PLGA-coated and uncoated β-TCP scaffolds. SEM analyses showed no relevant blood cell attachment and ELISA results revealed no significant increase in most of the analyzed cell activation markers (β-thromboglobulin, Sc5B-9, polymorphonuclear (PMN)-elastase). However, a notable increase in thrombin-antithrombin III (TAT) complex levels, as well as fibrin fiber accumulation on JPC-seeded β-TCP scaffolds, was detected compared to the scaffolds without JPCs. Thus, this study demonstrated that besides the scaffold material the cells colonizing the scaffolds can also influence hemostasis, which can influence the regeneration of bone tissue. View Full-Text
Keywords: jaw periosteal cells; bone tissue engineering; biomaterial; β-tricalcium phosphate; hemocompatibility jaw periosteal cells; bone tissue engineering; biomaterial; β-tricalcium phosphate; hemocompatibility
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MDPI and ACS Style

Weber, M.; Umrath, F.; Steinle, H.; Schmitt, L.-F.; Yu, L.T.; Schlensak, C.; Wendel, H.-P.; Reinert, S.; Alexander, D.; Avci-Adali, M. Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation. Int. J. Mol. Sci. 2021, 22, 9942. https://doi.org/10.3390/ijms22189942

AMA Style

Weber M, Umrath F, Steinle H, Schmitt L-F, Yu LT, Schlensak C, Wendel H-P, Reinert S, Alexander D, Avci-Adali M. Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation. International Journal of Molecular Sciences. 2021; 22(18):9942. https://doi.org/10.3390/ijms22189942

Chicago/Turabian Style

Weber, Marbod, Felix Umrath, Heidrun Steinle, Lukas-Frank Schmitt, Lin T. Yu, Christian Schlensak, Hans-Peter Wendel, Siegmar Reinert, Dorothea Alexander, and Meltem Avci-Adali. 2021. "Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation" International Journal of Molecular Sciences 22, no. 18: 9942. https://doi.org/10.3390/ijms22189942

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