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Article

Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA

1
Jerome Canady Research Institute for Advanced Biological and Technological Sciences, Takoma Park, MD 20912, USA
2
School of Medicine, University of Massachusetts, Worcester, MA 01605, USA
3
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052, USA
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Microbiology Department, Howard University, Washington, DC 20060, USA
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Howard University Cancer Center, Howard University, Washington, DC 20060, USA
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Department of Surgery, Holy Cross Hospital, Silver Spring, MD 20910, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Eva Horvathova
Int. J. Mol. Sci. 2021, 22(17), 9578; https://doi.org/10.3390/ijms22179578
Received: 28 July 2021 / Revised: 24 August 2021 / Accepted: 1 September 2021 / Published: 3 September 2021
(This article belongs to the Special Issue Non-thermal Plasma Interactions with Different Living Systems)
Breast cancer is the most common cancer among women worldwide. Its molecular receptor marker status and mutational subtypes complicate clinical therapies. Cold atmospheric plasma is a promising adjuvant therapy to selectively combat many cancers, including breast cancer, but not normal tissue; however, the underlying mechanisms remain unexplored. Here, four breast cancer cell lines with different marker status were treated with Canady Helios Cold Plasma™ (CHCP) at various dosages and their differential progress of apoptosis was monitored. Inhibition of cell proliferation, induction of apoptosis, and disruption of the cell cycle were observed. At least 16 histone mRNA types were oxidized and degraded immediately after CHCP treatment by 8-oxoguanine (8-oxoG) modification. The expression of DNA damage response genes was up-regulated 12 h post-treatment, indicating that 8-oxoG modification and degradation of histone mRNA during the early S phase of the cell cycle, rather than DNA damage, is the primary cause of cancer cell death induced by CHCP. Our report demonstrates for the first time that CHCP effectively induces cell death in breast cancer regardless of subtyping, through histone mRNA oxidation and degradation during the early S phase of the cell cycle. View Full-Text
Keywords: breast cancer; cold atmospheric plasma; cancer treatment; oxidation of RNA; histone gene breast cancer; cold atmospheric plasma; cancer treatment; oxidation of RNA; histone gene
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MDPI and ACS Style

Cheng, X.; Murthy, S.R.K.; Zhuang, T.; Ly, L.; Jones, O.; Basadonna, G.; Keidar, M.; Kanaan, Y.; Canady, J. Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA. Int. J. Mol. Sci. 2021, 22, 9578. https://doi.org/10.3390/ijms22179578

AMA Style

Cheng X, Murthy SRK, Zhuang T, Ly L, Jones O, Basadonna G, Keidar M, Kanaan Y, Canady J. Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA. International Journal of Molecular Sciences. 2021; 22(17):9578. https://doi.org/10.3390/ijms22179578

Chicago/Turabian Style

Cheng, Xiaoqian, Saravana R.K. Murthy, Taisen Zhuang, Lawan Ly, Olivia Jones, Giacomo Basadonna, Michael Keidar, Yasmine Kanaan, and Jerome Canady. 2021. "Canady Helios Cold Plasma Induces Breast Cancer Cell Death by Oxidation of Histone mRNA" International Journal of Molecular Sciences 22, no. 17: 9578. https://doi.org/10.3390/ijms22179578

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