Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets
Abstract
:1. Introduction
2. Results
2.1. Nec-1 Supplementation on Day 3 of Tissue Culture Improves Islet Recovery
2.2. Nec-1 Supplementation on Day 3 of Tissue Culture Enhances the Development of Islet Endocrine Cells and Upregulates the Expression of GLUT2 in Beta Cells
2.3. Nec-1 Supplementation on Day 3 of Tissue Culture Improves the Differentiation of Pancreatic Progenitor Cells
2.4. Nec-1 Supplementation on Day 3 of Tissue Culture Stimulates the Proliferation of Islet Endocrine Cells
2.5. Nec-1 Supplementation on Day 3 of Tissue Culture Increases Insulin Content and Glucose-Stimulated Insulin Secretion
2.6. Nec-1 Supplementation on Day 3 of Tissue Culture Improves Islet Function after Transplantation into STZ-Induced Diabetic Mice
3. Discussion
4. Research Design and Methods
4.1. Animals
4.2. PPI Isolation
4.3. Islet Tissue Culture and Nec-1 Supplementation
4.4. Islet Recovery and Viability
4.5. Flow Cytometric Analysis of Islets
4.6. In Vitro Islet Function
4.7. Islet Insulin Content
4.8. Islet Transplantation and Metabolic Follow-Up
4.9. Histological and Immunohistochemical Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lau, H.; Li, S.; Corrales, N.; Rodriguez, S.; Mohammadi, M.; Alexander, M.; de Vos, P.; Lakey, J.R. Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets. Int. J. Mol. Sci. 2021, 22, 8367. https://doi.org/10.3390/ijms22168367
Lau H, Li S, Corrales N, Rodriguez S, Mohammadi M, Alexander M, de Vos P, Lakey JR. Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets. International Journal of Molecular Sciences. 2021; 22(16):8367. https://doi.org/10.3390/ijms22168367
Chicago/Turabian StyleLau, Hien, Shiri Li, Nicole Corrales, Samuel Rodriguez, Mohammadreza Mohammadi, Michael Alexander, Paul de Vos, and Jonathan RT Lakey. 2021. "Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets" International Journal of Molecular Sciences 22, no. 16: 8367. https://doi.org/10.3390/ijms22168367