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Article

Reprogramming mRNA Expression in Response to Defect in RNA Polymerase III Assembly in the Yeast Saccharomyces cerevisiae

1
Laboratory of tRNA Transcription, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland
2
Wellcome Centre for Cell Biology, The University of Edinburgh, Edinburgh EH8 9YL, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Ciani
Int. J. Mol. Sci. 2021, 22(14), 7298; https://doi.org/10.3390/ijms22147298
Received: 31 May 2021 / Revised: 25 June 2021 / Accepted: 3 July 2021 / Published: 7 July 2021
The coordinated transcription of the genome is the fundamental mechanism in molecular biology. Transcription in eukaryotes is carried out by three main RNA polymerases: Pol I, II, and III. One basic problem is how a decrease in tRNA levels, by downregulating Pol III efficiency, influences the expression pattern of protein-coding genes. The purpose of this study was to determine the mRNA levels in the yeast mutant rpc128-1007 and its overdose suppressors, RBS1 and PRT1. The rpc128-1007 mutant prevents assembly of the Pol III complex and functionally mimics similar mutations in human Pol III, which cause hypomyelinating leukodystrophies. We applied RNAseq followed by the hierarchical clustering of our complete RNA-seq transcriptome and functional analysis of genes from the clusters. mRNA upregulation in rpc128-1007 cells was generally stronger than downregulation. The observed induction of mRNA expression was mostly indirect and resulted from the derepression of general transcription factor Gcn4, differently modulated by suppressor genes. rpc128-1007 mutation, regardless of the presence of suppressors, also resulted in a weak increase in the expression of ribosome biogenesis genes. mRNA genes that were downregulated by the reduction of Pol III assembly comprise the proteasome complex. In summary, our results provide the regulatory links affected by Pol III assembly that contribute differently to cellular fitness. View Full-Text
Keywords: yeast; gene expression; RNA polymerase yeast; gene expression; RNA polymerase
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MDPI and ACS Style

Rudzińska, I.; Cieśla, M.; Turowski, T.W.; Armatowska, A.; Leśniewska, E.; Boguta, M. Reprogramming mRNA Expression in Response to Defect in RNA Polymerase III Assembly in the Yeast Saccharomyces cerevisiae. Int. J. Mol. Sci. 2021, 22, 7298. https://doi.org/10.3390/ijms22147298

AMA Style

Rudzińska I, Cieśla M, Turowski TW, Armatowska A, Leśniewska E, Boguta M. Reprogramming mRNA Expression in Response to Defect in RNA Polymerase III Assembly in the Yeast Saccharomyces cerevisiae. International Journal of Molecular Sciences. 2021; 22(14):7298. https://doi.org/10.3390/ijms22147298

Chicago/Turabian Style

Rudzińska, Izabela, Małgorzata Cieśla, Tomasz W. Turowski, Alicja Armatowska, Ewa Leśniewska, and Magdalena Boguta. 2021. "Reprogramming mRNA Expression in Response to Defect in RNA Polymerase III Assembly in the Yeast Saccharomyces cerevisiae" International Journal of Molecular Sciences 22, no. 14: 7298. https://doi.org/10.3390/ijms22147298

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