Cyanine Dyes for Photo-Thermal Therapy: A Comparison of Synthetic Liposomes and Natural Erythrocyte-Based Carriers
Abstract
:1. Introduction
2. Results and Discussion
2.1. Light Absorption Properties of ICG and IR-820 in Different Solvents
2.2. Temperature-Stability Study of ICG and IR-820 in Different Solvents
2.3. Photo-Stability Study of ICG and IR-820 Solutions in PBS
2.4. Preparation of ICG- and IR-820-Loaded Carriers
2.5. Temperature Stability of Dye-Loaded Liposomes and EMVs
2.6. Photo-Thermal Effect of ICG- and IR-820-Loaded Liposomes and EMVs
2.7. Hemolysis Study
3. Materials and Methods
3.1. Chemicals
3.2. Preparation of Dye-Loaded Liposomes
3.3. Preparation of Dye-Loaded EMVs
3.4. Purification of Dye-Loaded EMVs/Liposomes and Encapsulation Efficiency
3.5. Hydrodynamic Diameter and Zeta Potential Measurements
3.6. Absorption Spectra of IR-820 and ICG Solutions
3.7. Temperature Stability of IR-820 and ICG Solutions
3.8. Photo-Stability of IR-820 and ICG Solutions
3.9. Hemotoxicity Study
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Concentration (μg/mL) | P (W/cm2) | Absorbance before Irradiation | Absorbance after Irradiation | % Degradation | ΔT (°C) |
---|---|---|---|---|---|
5 | 0.5 | 0.748 | 0.295 | 60.5 | 3.8 |
5 | 1.0 | 0.748 | 0.237 | 68.3 | 5.1 |
5 | 3.0 | 0.748 | 0.145 | 80.6 | 7.5 |
25 | 0.5 | 2.326 | 1.884 | 19.0 | 13.2 |
25 | 1.0 | 2.326 | 1.398 | 39.9 | 21.8 |
25 | 3.0 | 2.326 | 0.375 | 83.9 | 31.1 |
50 | 0.5 | 3.678 | 3.567 | 3.0 | 12.8 |
50 | 1.0 | 3.678 | 3.197 | 13.1 | 24.2 |
50 | 3.0 | 3.678 | 1.182 | 67.9 | 50.7 |
Concentration (μg/mL) | P (W/cm2) | Absorbance before Irradiation | Absorbance after Irradiation | % Degradation | ΔT (°C) |
---|---|---|---|---|---|
5 | 0.5 | 0.160 | 0.070 | 56.3 | 1.8 |
5 | 1.0 | 0.160 | 0.056 | 64.8 | 2.0 |
5 | 3.0 | 0.160 | 0.036 | 77.4 | 3.4 |
25 | 0.5 | 0.491 | 0.150 | 69.5 | 4.8 |
25 | 1.0 | 0.491 | 0.117 | 76.1 | 10.0 |
25 | 3.0 | 0.491 | 0.095 | 80.7 | 8.0 |
50 | 0.5 | 1.410 | 0.655 | 53.5 | 11.7 |
50 | 1.0 | 1.410 | 0.271 | 80.8 | 19.0 |
50 | 3.0 | 1.410 | 0.093 | 93.4 | 27.3 |
Sample | Size (nm) | PDI | EE (%) |
---|---|---|---|
Empty LPS | 115 ± 1 | 0.087 ± 0.050 | - |
Empty EMVs | 234 ± 4 | 0.238 ± 0.011 | - |
ICG LPS | 126 ± 3 | 0.171 ± 0.083 | 45 ± 3 |
ICG-EMVs | 215 ± 5 | 0.198 ± 0.075 | 57 ± 2 |
IR-820 LPS | 116 ± 2 | 0.142 ± 0.091 | 46 ± 4 |
IR-820 EMVs | 260 ± 4 | 0.229 ± 0.081 | 53 ± 3 |
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Della Pelle, G.; Delgado López, A.; Salord Fiol, M.; Kostevšek, N. Cyanine Dyes for Photo-Thermal Therapy: A Comparison of Synthetic Liposomes and Natural Erythrocyte-Based Carriers. Int. J. Mol. Sci. 2021, 22, 6914. https://doi.org/10.3390/ijms22136914
Della Pelle G, Delgado López A, Salord Fiol M, Kostevšek N. Cyanine Dyes for Photo-Thermal Therapy: A Comparison of Synthetic Liposomes and Natural Erythrocyte-Based Carriers. International Journal of Molecular Sciences. 2021; 22(13):6914. https://doi.org/10.3390/ijms22136914
Chicago/Turabian StyleDella Pelle, Giulia, Andrea Delgado López, Marina Salord Fiol, and Nina Kostevšek. 2021. "Cyanine Dyes for Photo-Thermal Therapy: A Comparison of Synthetic Liposomes and Natural Erythrocyte-Based Carriers" International Journal of Molecular Sciences 22, no. 13: 6914. https://doi.org/10.3390/ijms22136914