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Article

Satellitome Analysis of the Pacific Oyster Crassostrea gigas Reveals New Pattern of Satellite DNA Organization, Highly Scattered across the Genome

1
Division of Molecular Biology, Ruđer Bošković Institute, 10000 Zagreb, Croatia
2
Centro de Investigación Mariña, Universidade de Vigo, Dpto de Bioquímica, Xenética e Inmunoloxía, 36310 Vigo, Spain
3
Genomes and Disease, Centre for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Spain
4
Department of Zoology, Genetics and Physical Anthropology, Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Spain
5
Rimac Automobili d.o.o., Ljubljanska ulica 7, 10431 Sveta Nedelja, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Naoyuki Kataoka
Int. J. Mol. Sci. 2021, 22(13), 6798; https://doi.org/10.3390/ijms22136798
Received: 9 June 2021 / Revised: 18 June 2021 / Accepted: 19 June 2021 / Published: 24 June 2021
(This article belongs to the Special Issue Repetitive DNA Sequences in Eukaryotic Genomes)
Several features already qualified the invasive bivalve species Crassostrea gigas as a valuable non-standard model organism in genome research. C. gigas is characterized by the low contribution of satellite DNAs (satDNAs) vs. mobile elements and has an extremely low amount of heterochromatin, predominantly built of DNA transposons. In this work, we have identified 52 satDNAs composing the satellitome of C. gigas and constituting about 6.33% of the genome. Satellitome analysis reveals unusual, highly scattered organization of relatively short satDNA arrays across the whole genome. However, peculiar chromosomal distribution and densities are specific for each satDNA. The inspection of the organizational forms of the 11 most abundant satDNAs shows association with constitutive parts of Helitron mobile elements. Nine of the inspected satDNAs are dominantly found in mobile element-associated form, two mostly appear standalone, and only one is present exclusively as Helitron-associated sequence. The Helitron-related satDNAs appear in more chromosomes than other satDNAs, indicating that these mobile elements could be leading satDNA propagation in C. gigas. No significant accumulation of satDNAs on certain chromosomal positions was detected in C. gigas, thus establishing a novel pattern of satDNA organization on the genome level. View Full-Text
Keywords: satellite DNA; satellitome; mobile element; Helitron; bivalve; Crassostrea gigas satellite DNA; satellitome; mobile element; Helitron; bivalve; Crassostrea gigas
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MDPI and ACS Style

Tunjić-Cvitanić, M.; Pasantes, J.J.; García-Souto, D.; Cvitanić, T.; Plohl, M.; Šatović-Vukšić, E. Satellitome Analysis of the Pacific Oyster Crassostrea gigas Reveals New Pattern of Satellite DNA Organization, Highly Scattered across the Genome. Int. J. Mol. Sci. 2021, 22, 6798. https://doi.org/10.3390/ijms22136798

AMA Style

Tunjić-Cvitanić M, Pasantes JJ, García-Souto D, Cvitanić T, Plohl M, Šatović-Vukšić E. Satellitome Analysis of the Pacific Oyster Crassostrea gigas Reveals New Pattern of Satellite DNA Organization, Highly Scattered across the Genome. International Journal of Molecular Sciences. 2021; 22(13):6798. https://doi.org/10.3390/ijms22136798

Chicago/Turabian Style

Tunjić-Cvitanić, Monika, Juan J. Pasantes, Daniel García-Souto, Tonči Cvitanić, Miroslav Plohl, and Eva Šatović-Vukšić. 2021. "Satellitome Analysis of the Pacific Oyster Crassostrea gigas Reveals New Pattern of Satellite DNA Organization, Highly Scattered across the Genome" International Journal of Molecular Sciences 22, no. 13: 6798. https://doi.org/10.3390/ijms22136798

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