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Article

Hybrid Methacrylated Gelatin and Hyaluronic Acid Hydrogel Scaffolds. Preparation and Systematic Characterization for Prospective Tissue Engineering Applications

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Department of Chemical Engineering, CUCEI, Universidad de Guadalajara, Guadalajara 44430, Mexico
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Colloids and Polymers Physics Group, Department of Particle Physics, Faculty of Physics and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain
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Department of Pharmacology, Pharmacy and Pharmaceutical Technology, I + D Farma Group (GI-1645), Faculty of Pharmacy and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain
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Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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Biophysics and Interfaces Group, Department of Applied Physics, Faculty of Physics, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain
*
Authors to whom correspondence should be addressed.
Academic Editors: Artur J.M. Valente, Edvani C. Muniz, Pietro Matricardi and You-Lo Hsieh
Int. J. Mol. Sci. 2021, 22(13), 6758; https://doi.org/10.3390/ijms22136758
Received: 14 May 2021 / Revised: 17 June 2021 / Accepted: 19 June 2021 / Published: 23 June 2021
Hyaluronic acid (HA) and gelatin (Gel) are major components of the extracellular matrix of different tissues, and thus are largely appealing for the construction of hybrid hydrogels to combine the favorable characteristics of each biopolymer, such as the gel adhesiveness of Gel and the better mechanical strength of HA, respectively. However, despite previous studies conducted so far, the relationship between composition and scaffold structure and physico-chemical properties has not been completely and systematically established. In this work, pure and hybrid hydrogels of methacroyl-modified HA (HAMA) and Gel (GelMA) were prepared by UV photopolymerization and an extensive characterization was done to elucidate such correlations. Methacrylation degrees of ca. 40% and 11% for GelMA and HAMA, respectively, were obtained, which allows to improve the hydrogels’ mechanical properties. Hybrid GelMA/HAMA hydrogels were stiffer, with elastic modulus up to ca. 30 kPa, and porous (up to 91%) compared with pure GelMA ones at similar GelMA concentrations thanks to the interaction between HAMA and GelMA chains in the polymeric matrix. The progressive presence of HAMA gave rise to scaffolds with more disorganized, stiffer, and less porous structures owing to the net increase of mass in the hydrogel compositions. HAMA also made hybrid hydrogels more swellable and resistant to collagenase biodegradation. Hence, the suitable choice of polymeric composition allows to regulate the hydrogels´ physical properties to look for the most optimal characteristics required for the intended tissue engineering application. View Full-Text
Keywords: gelatin; hyaluronic acid; hydrogel; hybrid scaffolds; tissue engineering; porosity gelatin; hyaluronic acid; hydrogel; hybrid scaffolds; tissue engineering; porosity
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MDPI and ACS Style

Velasco-Rodriguez, B.; Diaz-Vidal, T.; Rosales-Rivera, L.C.; García-González, C.A.; Alvarez-Lorenzo, C.; Al-Modlej, A.; Domínguez-Arca, V.; Prieto, G.; Barbosa, S.; Soltero Martínez, J.F.A.; Taboada, P. Hybrid Methacrylated Gelatin and Hyaluronic Acid Hydrogel Scaffolds. Preparation and Systematic Characterization for Prospective Tissue Engineering Applications. Int. J. Mol. Sci. 2021, 22, 6758. https://doi.org/10.3390/ijms22136758

AMA Style

Velasco-Rodriguez B, Diaz-Vidal T, Rosales-Rivera LC, García-González CA, Alvarez-Lorenzo C, Al-Modlej A, Domínguez-Arca V, Prieto G, Barbosa S, Soltero Martínez JFA, Taboada P. Hybrid Methacrylated Gelatin and Hyaluronic Acid Hydrogel Scaffolds. Preparation and Systematic Characterization for Prospective Tissue Engineering Applications. International Journal of Molecular Sciences. 2021; 22(13):6758. https://doi.org/10.3390/ijms22136758

Chicago/Turabian Style

Velasco-Rodriguez, B., T. Diaz-Vidal, L. C. Rosales-Rivera, C. A. García-González, C. Alvarez-Lorenzo, A. Al-Modlej, V. Domínguez-Arca, G. Prieto, S. Barbosa, J. F. A. Soltero Martínez, and P. Taboada. 2021. "Hybrid Methacrylated Gelatin and Hyaluronic Acid Hydrogel Scaffolds. Preparation and Systematic Characterization for Prospective Tissue Engineering Applications" International Journal of Molecular Sciences 22, no. 13: 6758. https://doi.org/10.3390/ijms22136758

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