A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA
1
Department of Biological Sciences, University of Maryland Baltimore County (UMBC), 1000 Hilltop Circle, Baltimore, MD 21250, USA
2
Department of Biology, University of Rochester, Rochester, NY 14627, USA
*
Author to whom correspondence should be addressed.
†
Current address: Abbott Laboratories, San Diego, CA 92121, USA.
Academic Editor: Yves Henry
Int. J. Mol. Sci. 2021, 22(13), 6690; https://doi.org/10.3390/ijms22136690
Received: 27 April 2021
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Revised: 14 June 2021
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Accepted: 17 June 2021
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Published: 22 June 2021
(This article belongs to the Special Issue Ribosome Biogenesis in “War and Peace of the Cell”)
Processing of the RNA polymerase I pre-rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs requires removing the “spacer” sequences. The canonical pathway for the removal of the ITS1 spacer involves cleavages at the 3′ end of 18S rRNA and at two sites inside ITS1. The process can generate either a long or a short 5.8S rRNA that differs in the number of ITS1 nucleotides retained at the 5.8S 5′ end. Here we document a novel pathway to the long 5.8S, which bypasses cleavage within ITS1. Instead, the entire ITS1 is degraded from its 5′ end by exonuclease Xrn1. Mutations in RNase MRP increase the accumulation of long relative to short 5.8S rRNA. Traditionally this is attributed to a decreased rate of RNase MRP cleavage at its target in ITS1, called A3. However, results from this work show that the MRP-induced switch between long and short 5.8S rRNA formation occurs even when the A3 site is deleted. Based on this and our published data, we propose that the link between RNase MRP and 5.8S 5′ end formation involves RNase MRP cleavage at unknown sites elsewhere in pre-rRNA or in RNA molecules other than pre-rRNA.
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Keywords:
ribosome biogenesis; rRNA processing; RNase MRP; long/short 5.8S rRNA
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MDPI and ACS Style
Li, X.; Zengel, J.M.; Lindahl, L. A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA. Int. J. Mol. Sci. 2021, 22, 6690. https://doi.org/10.3390/ijms22136690
AMA Style
Li X, Zengel JM, Lindahl L. A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA. International Journal of Molecular Sciences. 2021; 22(13):6690. https://doi.org/10.3390/ijms22136690
Chicago/Turabian StyleLi, Xiao, Janice M. Zengel, and Lasse Lindahl. 2021. "A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA" International Journal of Molecular Sciences 22, no. 13: 6690. https://doi.org/10.3390/ijms22136690
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