A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA
Abstract
:1. Introduction
2. Results
2.1. System for Genetic Analysis
2.2. Effects of ITS1 Deletions on 5.8S rRNA Processing
2.3. pre-rRNA Processing Is Altered by an RNase MRP Mutation Even When the ITS1 A3 Site Is Deleted
2.4. A Pathway to 5.8S rRNA That Bypasses the Canonical A2 and A3 Cleavage Sites in ITS1
2.5. 5.8S rRNA with 5′ Extended Ends Are Incorporated into Functional Ribosomes
3. Discussion
3.1. Steps in the Xrn1-Dependent Path to the 5′ End of 5.8SL
3.2. The RNase MRP-Induced Switch between 5.8SL and 5.8SS Production Not Due to Changes in A3 Cleavage
3.3. The 3′ End of ITS1 Facilitates the Xrn1-Mediated Processing
3.4. The 3′ End Maturation of 18S rRNA
3.5. Diversity of rRNA Processing Pathways
4. Materials and Methods
4.1. Strains and Growth Conditions
4.2. Plasmids and Oligonucleotides
4.3. Other Procedures
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Oligo | Sequence (5’→3’) | Complementary to |
---|---|---|
O9 | GCT CTT GCC AAA ACA AAA AAA TCC A | ITS1 24–53 |
O20 | AAA TGA CGC TCA AAC AGG CAT GCC C | 5.8S 3′ end |
O90 | GTA TCA CTC ACT ACC AAA CAG AAT G | ITS2 11–35 |
O453 | AAC AAA AAA ATC CAT TTT CAA | ITS1 15–25 |
O552 | CCA GTT ACG CGT TTT AAT TG | Spanning ∆2 deletion, see text |
O553 | ATG CCT TTG GTA GAA CCC AAA GGC | S. pombe hairpin inserted in S. cerevisiae 5.8S |
O576 | ATG CCT TTG GTA GAA CCC | S. pombe hairpin inserted in S. cerevisiae 5.8S |
Name | Genotype | References |
---|---|---|
∆rpa12 alias NOY504 | MATa rm4 (rpa12)::LEU2 ade2-101 ura3-1 trp1-1 leu2-3,112 his3-101 can1-100 | [34] |
∆rpa135 alias NOY408-la | MATa rpa135::LEU2 ade2-1 ura3-1 his3-10 1trp1-1 leu2-112 can1-100/pNOY102 | [33] |
∆rpa135/pDK16 | MATa rpa135::LEU2 ade2-l ura3-l his3-ll trpl-1 leu2-3,112 can1-100/pDK16 | This study |
YLL53 | MATa ade2-101 his3∆200 ura3-52tyr1RRP2 | [23] |
YLL54 | MATa ade2-101 his3∆200 ura3-52, lys2, rrp2-2 | [23] |
pDK16 | YEplac112 carrying the rRNA transcription unit expressed from the CUP1 promoter | [35] |
pDK16-tag | pDK16 with tagged 5,8S gene (see text) | This study |
ITS1 Nucleotides | L-Fraction | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Mutant | A2 | A3 | 283–289 | 290–299 | 300–330 | 331–340 | ∆rpa12 ∆xrn1 | RRP2 (YLL53) | HI mw interm in ∆rpa12 ∆xrn1 | RRP2 sensitivity |
wt | yes | yes | P | P | P | P | 0.21 | 0.20 | − | + |
∆13 | no | yes | P | P | P | P | 0.19 | 0.21 | − | + |
∆14 | no | no | A | A | P | P | 0.31 | 0.34 | − | + |
∆15 | yes | no | A | A | P | P | 0.26 | 0.40 | − | + |
∆16 | yes | yes | P | P | P | P | 0.22 | 0.14 | − | + |
∆17 | yes | yes | P | P | A | A | 0.61 | 0.88 | + | − |
∆18 | yes | half | P | A | P | P | 0.50 | 0.80 | + | − |
∆19 | yes | no | A | A | A | P | 0.56 | 0.64 | − | nd |
∆2 | no | no | A | A | A | A | 0.74 | 0.82 | + | − |
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Li, X.; Zengel, J.M.; Lindahl, L. A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA. Int. J. Mol. Sci. 2021, 22, 6690. https://doi.org/10.3390/ijms22136690
Li X, Zengel JM, Lindahl L. A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA. International Journal of Molecular Sciences. 2021; 22(13):6690. https://doi.org/10.3390/ijms22136690
Chicago/Turabian StyleLi, Xiao, Janice M. Zengel, and Lasse Lindahl. 2021. "A Novel Model for the RNase MRP-Induced Switch between the Formation of Different Forms of 5.8S rRNA" International Journal of Molecular Sciences 22, no. 13: 6690. https://doi.org/10.3390/ijms22136690