The Role of Biodegradable Poly-(L-lactide)-Based Polymers in Blood Cell Activation and Platelet-Monocyte Interaction
Abstract
:1. Introduction
2. Results
2.1. Polymer Wettability
2.2. Platelet–Polymer Interaction
2.3. Leukocyte–Polymer Interaction
2.4. Monocyte Activation
2.5. Platelet–Monocyte Aggregation
3. Discussion
3.1. Platelet–Polymer Interactions Are Influenced by Local Hemodynamic Conditions
3.2. Biomaterials Facilitate Leucocyte–Polymer Interactions
3.3. Biomaterials Influence the Formation of CD62P+ Monocyte-Bound Platelets (MBPs)
4. Materials and Methods
4.1. Polymer Film Preparation
4.2. Surface Characterization
4.3. Isolation of Human Blood Cells
4.4. Flow Experiments
4.5. Platelet Aggregation
4.6. Measurement of Surface P-Selectin and Plasma Fibrinogen (Fg)
4.7. Leukocyte Tracking
4.8. Monocyte Adhesion
4.9. Flow Cytometry for the Detection of Monocytes Activation and Platelet–Monocyte Aggregates
4.10. Data Collection and Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Polymer | Θ W [°] Mean±Standard Deviation | p-Value |
---|---|---|
P(4HB) | 72.1 ± 3.9 | <0.001 |
PLLA/P(4HB) | 76.7 ± 2.8 | 0.001 |
PCL | 78.6 ± 3.2 | 0.043 |
PLLA/PCL | 79.9 ± 3.4 | 0.295 |
PLLA | 82.2 ± 1.8 |
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Strohbach, A.; Maess, F.; Wulf, K.; Petersen, S.; Grabow, N.; Schmitz, K.-P.; Felix, S.B.; Busch, R. The Role of Biodegradable Poly-(L-lactide)-Based Polymers in Blood Cell Activation and Platelet-Monocyte Interaction. Int. J. Mol. Sci. 2021, 22, 6340. https://doi.org/10.3390/ijms22126340
Strohbach A, Maess F, Wulf K, Petersen S, Grabow N, Schmitz K-P, Felix SB, Busch R. The Role of Biodegradable Poly-(L-lactide)-Based Polymers in Blood Cell Activation and Platelet-Monocyte Interaction. International Journal of Molecular Sciences. 2021; 22(12):6340. https://doi.org/10.3390/ijms22126340
Chicago/Turabian StyleStrohbach, Anne, Friedemann Maess, Katharina Wulf, Svea Petersen, Niels Grabow, Klaus-Peter Schmitz, Stephan B. Felix, and Raila Busch. 2021. "The Role of Biodegradable Poly-(L-lactide)-Based Polymers in Blood Cell Activation and Platelet-Monocyte Interaction" International Journal of Molecular Sciences 22, no. 12: 6340. https://doi.org/10.3390/ijms22126340