The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana
Abstract
:1. Introduction
2. Results
2.1. BAG2 and BAG6 Expression during Arabidopsis Development
2.2. BAG2 and BAG6 Expression Responses to Abiotic Stress and Plant Hormones
2.3. BAG2 and BAG6 Genes Are Involved in Arabidopsis Responses to ABA and Drought Treatment
2.4. Mutation of BAG2 and BAG6 in Arabidopsis Compromises Tolerance to Heat Stress
2.5. Stress- and ABA-Related Genes Are Differentially Regulated in the bag2, bag6, and bag2 bag6 Mutants Compared to WT
2.6. ROS Accumulation in WT and Mutant Plants
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Vector Construction and Generation of Transgenic Plants
4.3. RT-PCR Analysis
4.4. GUS Histochemical Staining
4.5. Drought and ABA Treatments
4.6. Heat Treatment and Measurement of Chlorophyll Content
4.7. DAB and NBT Staining
4.8. Hormone Treatment
4.9. RT-qPCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
BAG | Bcl-2 associated athanogene |
ABA | Abscisic acid |
ACC | 1-aminocyclopropane-1-carboxylic acid |
JA | Jasmonate |
SA | Salicylic acid |
GA | Gibberellic acid |
WT | Wild type |
GUS | β-glucuronidase |
PCD | programmed cell death |
ROS | reactive oxygen species |
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Arif, M.; Li, Z.; Luo, Q.; Li, L.; Shen, Y.; Men, S. The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana. Int. J. Mol. Sci. 2021, 22, 5856. https://doi.org/10.3390/ijms22115856
Arif M, Li Z, Luo Q, Li L, Shen Y, Men S. The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana. International Journal of Molecular Sciences. 2021; 22(11):5856. https://doi.org/10.3390/ijms22115856
Chicago/Turabian StyleArif, Muhammad, Zitong Li, Qiong Luo, Luhua Li, Yuequan Shen, and Shuzhen Men. 2021. "The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana" International Journal of Molecular Sciences 22, no. 11: 5856. https://doi.org/10.3390/ijms22115856