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Article

One Week of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver

1
Laboratory of Laboratory/Sports Medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
2
Master’s Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
3
Research and Development Section, Anicom Specialty Medical Institute Inc., 2-6-3 Chojamachi 5F, Yokohamashi-Nakaku 231-0033, Kanagawa, Japan
4
Japan Society for the Promotion of Science, Kojimachi Business Center Building, Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan
5
Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8577, Ibaraki, Japan
6
Department of Medical Technology, Faculty of Health Sciences, Tsukuba International University, 6-20-1 Manabe, Tsuchiura 300-0051, Ibaraki, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Walter Wahli
Int. J. Mol. Sci. 2021, 22(11), 5851; https://doi.org/10.3390/ijms22115851
Received: 4 May 2021 / Revised: 20 May 2021 / Accepted: 26 May 2021 / Published: 29 May 2021
(This article belongs to the Section Molecular Toxicology)
The prevalence of nonalcoholic fatty liver disease (NAFLD) has been rapidly increasing worldwide. A choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) has been used to create a mouse model of nonalcoholic steatohepatitis (NASH). There are some reports on the effects on mice of being fed a CDAHFD for long periods of 1 to 3 months. However, the effect of this diet over a short period is unknown. Therefore, we examined the effect of 1-week CDAHFD feeding on the mouse liver. Feeding a CDAHFD diet for only 1-week induced lipid droplet deposition in the liver with increasing activity of liver-derived enzymes in the plasma. On the other hand, it did not induce fibrosis or cirrhosis. Additionally, it was demonstrated that CDAHFD significantly impaired mitochondrial respiration with severe oxidative stress to the liver, which is associated with a decreasing mitochondrial DNA copy number and complex proteins. In the gene expression analysis of the liver, inflammatory and oxidative stress markers were significantly increased by CDAHFD. These results demonstrated that 1 week of feeding CDAHFD to mice induces steatohepatitis with mitochondrial dysfunction and severe oxidative stress, without fibrosis, which can partially mimic the early stage of NASH in humans. View Full-Text
Keywords: CDAHFD; NASH; mitochondrial dysfunction; liver; oxidative stress CDAHFD; NASH; mitochondrial dysfunction; liver; oxidative stress
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MDPI and ACS Style

Sugasawa, T.; Ono, S.; Yonamine, M.; Fujita, S.-i.; Matsumoto, Y.; Aoki, K.; Nakano, T.; Tamai, S.; Yoshida, Y.; Kawakami, Y.; Takekoshi, K. One Week of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver. Int. J. Mol. Sci. 2021, 22, 5851. https://doi.org/10.3390/ijms22115851

AMA Style

Sugasawa T, Ono S, Yonamine M, Fujita S-i, Matsumoto Y, Aoki K, Nakano T, Tamai S, Yoshida Y, Kawakami Y, Takekoshi K. One Week of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver. International Journal of Molecular Sciences. 2021; 22(11):5851. https://doi.org/10.3390/ijms22115851

Chicago/Turabian Style

Sugasawa, Takehito, Seiko Ono, Masato Yonamine, Shin-ichiro Fujita, Yuki Matsumoto, Kai Aoki, Takuro Nakano, Shinsuke Tamai, Yasuko Yoshida, Yasushi Kawakami, and Kazuhiro Takekoshi. 2021. "One Week of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver" International Journal of Molecular Sciences 22, no. 11: 5851. https://doi.org/10.3390/ijms22115851

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