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Article

Characterization of an Endolysin Targeting Clostridioides difficile That Affects Spore Outgrowth

1
APC Microbiome Ireland, University College Cork, T12 YT20 Cork, Ireland
2
Genetic Engineering and Biotechnology Department, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh
3
Biochemistry and Molecular Biology Department, Shahjalal University of Science and Technology, Sylhet 3114, Bangladesh
4
School of Microbiology, University College Cork, T12 K8AF Cork, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Alicja Wegrzyn
Int. J. Mol. Sci. 2021, 22(11), 5690; https://doi.org/10.3390/ijms22115690
Received: 12 April 2021 / Revised: 23 May 2021 / Accepted: 24 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Bacteriophage—Molecular Studies 3.0)
Clostridioides difficile is a spore-forming enteric pathogen causing life-threatening diarrhoea and colitis. Microbial disruption caused by antibiotics has been linked with susceptibility to, and transmission and relapse of, C. difficile infection. Therefore, there is an urgent need for novel therapeutics that are effective in preventing C. difficile growth, spore germination, and outgrowth. In recent years bacteriophage-derived endolysins and their derivatives show promise as a novel class of antibacterial agents. In this study, we recombinantly expressed and characterized a cell wall hydrolase (CWH) lysin from C. difficile phage, phiMMP01. The full-length CWH displayed lytic activity against selected C. difficile strains. However, removing the N-terminal cell wall binding domain, creating CWH351—656, resulted in increased and/or an expanded lytic spectrum of activity. C. difficile specificity was retained versus commensal clostridia and other bacterial species. As expected, the putative cell wall binding domain, CWH1—350, was completely inactive. We also observe the effect of CWH351—656 on preventing C. difficile spore outgrowth. Our results suggest that CWH351—656 has therapeutic potential as an antimicrobial agent against C. difficile infection. View Full-Text
Keywords: Clostridioides difficile infection; endolysin; antimicrobial agent; antimicrobial resistance; enteric pathogen; antibiotics Clostridioides difficile infection; endolysin; antimicrobial agent; antimicrobial resistance; enteric pathogen; antibiotics
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MDPI and ACS Style

Mondal, S.I.; Akter, A.; Draper, L.A.; Ross, R.P.; Hill, C. Characterization of an Endolysin Targeting Clostridioides difficile That Affects Spore Outgrowth. Int. J. Mol. Sci. 2021, 22, 5690. https://doi.org/10.3390/ijms22115690

AMA Style

Mondal SI, Akter A, Draper LA, Ross RP, Hill C. Characterization of an Endolysin Targeting Clostridioides difficile That Affects Spore Outgrowth. International Journal of Molecular Sciences. 2021; 22(11):5690. https://doi.org/10.3390/ijms22115690

Chicago/Turabian Style

Mondal, Shakhinur I., Arzuba Akter, Lorraine A. Draper, R. P. Ross, and Colin Hill. 2021. "Characterization of an Endolysin Targeting Clostridioides difficile That Affects Spore Outgrowth" International Journal of Molecular Sciences 22, no. 11: 5690. https://doi.org/10.3390/ijms22115690

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