Next Article in Journal
HNRNP A1 Promotes Lung Cancer Cell Proliferation by Modulating VRK1 Translation
Previous Article in Journal
Insight into Bortezomib Focusing on Its Efficacy against P-gp-Positive MDR Leukemia Cells
Previous Article in Special Issue
Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro
Article

The Effects of Insulin on Immortalized Rat Schwann Cells, IFRS1

1
Department of Pharmacy, Aichi Gakuin University Dental Hospital, Nagoya 464-8651, Japan
2
Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
3
Department of Clinical Laboratory, Aichi Gakuin University Dental Hospital, Nagoya 464-8651, Japan
4
Diabetic Neuropathy Project, Department of Diseases and Infection, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Elek Molnár and Louis Premkumar
Int. J. Mol. Sci. 2021, 22(11), 5505; https://doi.org/10.3390/ijms22115505
Received: 14 March 2021 / Revised: 18 May 2021 / Accepted: 19 May 2021 / Published: 23 May 2021
Schwann cells play an important role in peripheral nerve function, and their dysfunction has been implicated in the pathogenesis of diabetic neuropathy and other demyelinating diseases. The physiological functions of insulin in Schwann cells remain unclear and therefore define the aim of this study. By using immortalized adult Fischer rat Schwann cells (IFRS1), we investigated the mechanism of the stimulating effects of insulin on the cell proliferation and expression of myelin proteins (myelin protein zero (MPZ) and myelin basic protein (MBP). The application of insulin to IFRS1 cells increased the proliferative activity and induced phosphorylation of Akt and ERK, but not P38-MAPK. The proliferative potential of insulin-stimulated IFRS1 was significantly suppressed by the addition of LY294002, a PI3 kinase inhibitor. The insulin-stimulated increase in MPZ expression was significantly suppressed by the addition of PD98059, a MEK inhibitor. Furthermore, insulin-increased MBP expression was significantly suppressed by the addition of LY294002. These findings suggest that both PI3-K/Akt and ERK/MEK pathways are involved in insulin-induced cell growth and upregulation of MPZ and MBP in IFRS1 Schwann cells. View Full-Text
Keywords: Schwann cells; insulin; myelin protein zero; myelin basic protein; myelin associated glycoprotein; Akt; extracellular-signal-regulated kinase (ERK) Schwann cells; insulin; myelin protein zero; myelin basic protein; myelin associated glycoprotein; Akt; extracellular-signal-regulated kinase (ERK)
Show Figures

Figure 1

MDPI and ACS Style

Saiki, T.; Nakamura, N.; Miyabe, M.; Ito, M.; Minato, T.; Sango, K.; Matsubara, T.; Naruse, K. The Effects of Insulin on Immortalized Rat Schwann Cells, IFRS1. Int. J. Mol. Sci. 2021, 22, 5505. https://doi.org/10.3390/ijms22115505

AMA Style

Saiki T, Nakamura N, Miyabe M, Ito M, Minato T, Sango K, Matsubara T, Naruse K. The Effects of Insulin on Immortalized Rat Schwann Cells, IFRS1. International Journal of Molecular Sciences. 2021; 22(11):5505. https://doi.org/10.3390/ijms22115505

Chicago/Turabian Style

Saiki, Tomokazu, Nobuhisa Nakamura, Megumi Miyabe, Mizuho Ito, Tomomi Minato, Kazunori Sango, Tatsuaki Matsubara, and Keiko Naruse. 2021. "The Effects of Insulin on Immortalized Rat Schwann Cells, IFRS1" International Journal of Molecular Sciences 22, no. 11: 5505. https://doi.org/10.3390/ijms22115505

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop