UVB Inhibits Proliferation, Cell Cycle and Induces Apoptosis via p53, E2F1 and Microtubules System in Cervical Cancer Cell Lines
, Julio Enrique Castañeda-Delgado 4, Ixamail Fraire-Soto 1, Claudia Araceli Reyes-Estrada 5, Rosalinda Gutiérrez-Hernández 6 and Jesús Adrián López 1,*Round 1
Reviewer 1 Report
Please see the attachment.
Comments for author File: 
Comments.pdf
Author Response
We grateful thank you for your suggestions and observations.
We tried to address all issues, comments and suggestions that you kindly made in the review report.
- Please prepare a graphical abstract or summary for the manuscript and include it as the last figure.
Author´s response:
A graphical abstract was made.
- Write a specific conclusion.
Author´s response:
Specific conclusion was made.
- Line 9, [email protected]_(J.A.L.). Please check.
Author´s response:
The dash was deleted.
- Line 43, global incidence of 604 127 new cases and over 341 831 deaths. There is a space.
Author´s response:
The spaces were deleted.
- Line 52, being its effects dose, better write as effective dose.
Author´s response:
Suggestion was added.
- Make all boxes the same size and same configuration for a better look. Also mention what is happening in the figure for better illustration. The text is too small. Please improve.
Author´s response:
The figure was revised and all the boxes are in the same size and configuration.
- Line 139, written as G2, but line 141, G2. Please write similarly.
Author´s response:
It was corrected.
- Line 153, On their side, please revise.
Author´s response:
On their side was changed for On other hand.
- The text in figure 3a-g is very small and difficult to see. Please improve the figures. Mention the 4 sub-divisions of each box. The left panel is too small, while the right panel is too big. Adjust them in a way to look similar.
Author´s response:
Figure 3a-g was adjusted to bigger format.
The four sub-divisions were mentioned in the footnote of the figure.
The left and right panel were adjusted to look similar.
- Line 164, (g) SiHa cells were treated with increasing doses of UVB and apoptosis… seems that SiHa cells were treated with UVB and apoptosis. Please revise.
Author´s response:
This sentence was changed for “. Apoptosis and necrosis were analyzed by flow cytometry after 72 hours of incubation in (a) C-33A, (c) CaLo, (e) HaCaT and (g) SiHa cells treated with increasing doses of UVB”
- Line 167, A one-way ANOVA multiple comparison test with Tukey post-test was performed. Please correct.
Author´s response:
The sentence was corrected as fallows “A one-way ANOVA multiple comparison with Tukey post-hoc test was performed”.
- Figure 4, western blot image not so clear.
Author´s response:
The western blots images were improved.
- Line 241, These proteins regulation as also observed… Please revise.
Author´s response:
We add a w that was missing, “These proteins regulation was also observed”
- Line 299, was recovered with 10% acid acetic to be analyzed…. Please check.
Author´s response:
Acid acetic was changed to acetic acid.
- Line 302, at 20°C overnight. Please confirm 20°C or -20°C.
Author’s response:
-20°C is the correct temperature.
- Line 320, Detection was made using Western Blotting Luminol Reagent sc-2048.. Please mention the apparatus or machine used (i.e., film etc
Author´s response:
We added the program and the equipment used “Detection was made using Western Blotting Luminol Reagent sc-2048 (Santa Cruz Biotechnology, Texas, USA.) and analyzed in the Imagelab Software of Gel Doc TM XR ChemiDoc TM XRS, Bio-rad, Universal Hood II, USA.”
Reviewer 2 Report
An interesting original article about the use of UVB in immortal and tumorigenic HPV cells, showing the effectiveness in vitro of UVB on cervical cell cancer, suggesting that E2F1 and MAP2 are proteins that early respond to UVB while MAP1S, p53 and tubulins are proteins that play a role later in UVB induced signaling and cell damage.
Only minor queries before publication:
A conclusion paragraph, resuming the new knowledge obtained from this study and the possible future prospects with the results derived from this study would be a great addition to this paper.
Thank You
Author Response
We grateful thank you for your suggestions and observations.
A conclusion paragraph was added the manuscript.
Round 2
Reviewer 1 Report
Now the manuscript is much better. Please see the attachment for further comments.
Comments for author File: Comments.pdf
