Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade

Rahman, Md Saifur; Alam, Md Badrul; Kim, Young Kyun; Madina, Mst Hur; Fliss, Ismail; Lee, Sang Han; Yoo, Jin Cheol

doi:10.3390/ijms22105161

Open AccessArticle

Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade

by

Md Saifur Rahman

^1,2,†

,

Md Badrul Alam

^3,†

,

Young Kyun Kim

¹,

Mst Hur Madina

⁴,

Ismail Fliss

²,

Sang Han Lee

^3,*

and

Jin Cheol Yoo

^1,*

¹

Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501-759, Korea

²

Department of Food Science, Faculty of Agriculture and Food Sciences, Laval University, Québec, QC G1V 0A6, Canada

³

Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Korea

⁴

Department of Phytology, Faculty of Agriculture and Food Sciences, Laval University, Québec, QC G1V 0A6, Canada

^*

Authors to whom correspondence should be addressed.

^†

M.S.R. and M.B.A. contributed equally.

Int. J. Mol. Sci. 2021, 22(10), 5161; https://doi.org/10.3390/ijms22105161

Submission received: 14 April 2021 / Revised: 10 May 2021 / Accepted: 10 May 2021 / Published: 13 May 2021

(This article belongs to the Special Issue Pro- and Anti-inflammation Signaling Pathways and Development of Anti-inflammatory Drugs)

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Abstract

In this study, we investigate the immunomodulatory effects of a novel antimicrobial peptide, YD1, isolated from Kimchi, in both in vitro and in vivo models. We establish that YD1 exerts its anti-inflammatory effects via up-regulation of the Nrf2 pathway, resulting in the production of HO-1, which suppresses activation of the NF-κB pathway, including the subsequent proinflammatory cytokines IL-1β, IL-6, and TNF-α. We also found that YD1 robustly suppresses nitric oxide (NO) and prostaglandin E2 (PGE₂) production by down-regulating the expression of the upstream genes, iNOS and COX-2, acting as a strong antioxidant. Collectively, YD1 exhibits vigorous anti-inflammatory and antioxidant activity, presenting it as an interesting potential therapeutic agent.

Keywords: anti-inflammation; YD1; peptide drug; NF-κB; RAW 264.7; TLR-4; MyD88; AKT

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MDPI and ACS Style

Rahman, M.S.; Alam, M.B.; Kim, Y.K.; Madina, M.H.; Fliss, I.; Lee, S.H.; Yoo, J.C. Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade. Int. J. Mol. Sci. 2021, 22, 5161. https://doi.org/10.3390/ijms22105161

AMA Style

Rahman MS, Alam MB, Kim YK, Madina MH, Fliss I, Lee SH, Yoo JC. Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade. International Journal of Molecular Sciences. 2021; 22(10):5161. https://doi.org/10.3390/ijms22105161

Chicago/Turabian Style

Rahman, Md Saifur, Md Badrul Alam, Young Kyun Kim, Mst Hur Madina, Ismail Fliss, Sang Han Lee, and Jin Cheol Yoo. 2021. "Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade" International Journal of Molecular Sciences 22, no. 10: 5161. https://doi.org/10.3390/ijms22105161

APA Style

Rahman, M. S., Alam, M. B., Kim, Y. K., Madina, M. H., Fliss, I., Lee, S. H., & Yoo, J. C. (2021). Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade. International Journal of Molecular Sciences, 22(10), 5161. https://doi.org/10.3390/ijms22105161

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Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade

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