Exposure to 2.45 GHz Radiation Triggers Changes in HSP-70, Glucocorticoid Receptors and GFAP Biomarkers in Rat Brain
Abstract
:1. Introduction
2. Results
2.1. Heat Shock Protein (HSP) 70 Expression
2.1.1. HSP-70 in the Somatosensory Cortex
2.1.2. HSP-70 in the Limbic Cortex
2.1.3. HSP-70 in the Hypothalamus
2.1.4. HSP-70 in the Hippocampus
2.2. Glucocorticoid Receptor (GCR) Expression
2.2.1. GCR in the Somatosensory Cortex
2.2.2. GCR in the Limbic Cortex
2.2.3. GCR in the Hypothalamus
2.2.4. GCR in the Hippocampus
2.3. Glial Fibrillary Acidic Protein (GFAP) Expression
2.3.1. GFAP in the Somatosensory Cortex
2.3.2. GFAP in the Limbic Cortex
2.3.3. GFAP in the Hypothalamus
2.3.4. GFAP in the Hippocampus
3. Discussion
3.1. Influence and Temporal Evolution of 2.45 GHz Radiation on HSP-70 and GCR in Brain Tissues
3.2. The Influence and Temporal Evolution of 2.45 GHz Radiation on GFAP with the Decrease of GCR/HSP-70
4. Material and Methods
4.1. Experimental Radiation System Protocol
4.2. Experimental Design
4.2.1. Tissue Extraction and Preparation of Cell Extracts for the ELISA Technique
Determination of Protein Concentration
Chemiluminescent Enzyme-Linked Immunosorbent Assay (CLIA ELISA)
4.2.2. Morphological Studies with Immunohistochemistry
Perfusion and Tissue Processing
Immunohistochemistry
4.2.3. Quantification and Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Single Exposure (after 1.5 h) | Single Exposure (after 24 h) | Repeated Exposure | ||
---|---|---|---|---|
SMS CORTEX | NO RAD | 129 ± 16 b,c | 42 ± 11 a | 29 ± 5 a |
RAD | 112 ± 18 b,c | 37 ± 9 a | 39 ± 6 a | |
L. CORTEX | NO RAD | 123 ± 16 b,c | 34 ± 2 a | 26 ± 5 a |
RAD | 99 ± 18 b,c | 64 ± 13 a | 66 ± 22 *,a | |
HIPOTHA | NO RAD | 114 ± 20 b,c | 53 ± 7 a,c | 34 ± 11 a,b |
RAD | 117 ± 15 b,c | 46 ± 14 a | 45 ± 5 a | |
HIPPCAM | NO RAD | 179 ± 30 b,c | 89 ± 17 a,c | 37 ± 7 a,b |
RAD | 90 ± 8 *,c | 60 ± 20 | 41 ± 6 a |
Single Exposure (after 1.5 h) | Single Exposure (after 24 h) | Repeated Exposure | ||
---|---|---|---|---|
SMS CORTEX | NO RAD | 89 ± 24 c | 45 ± 10 | 35 ± 6 a |
RAD | 47 ± 14 *,c | 62 ± 17 | 30 ± 4 a | |
L. CORTEX | NO RAD | 174 ± 7 b,c | 233 ± 7 a,c | 115 ± 8 a,b |
RAD | 90 ± 7 *,b,c | 141 ± 9 *a | 167 ± 9 *a | |
HIPOTHA | NO RAD | 95 ± 16 b,c | 18 ± 10 a,c | 50 ± 11 a,b |
RAD | 50 ± 8 * | 14 ± 9 | 23 ± 4 | |
HIPPCAM | NO RAD | 91 ± 15 b,c | 49 ± 11 a | 42 ± 7 a |
RAD | 78 ± 14 b | 38 ± 13 a | 41 ± 4 a |
Single Exposure (after 1.5 h) | Single Exposure (after 24 h) | Repeated Exposure | ||
---|---|---|---|---|
SMS CORTEX | NO RAD | 30 ± 2 b | 54 ± 5 a | 29 ± 5 |
RAD | 28 ± 3 b | 48 ± 10 a | 39 ± 6 | |
L. CORTEX | NO RAD | 35 ± 3 b,c | 53 ± 7 a,c | 260 ± 27 a,b |
RAD | 42 ± 5 c | 57 ± 5 c | 175 ± 20 *,b | |
HIPOTHA | NO RAD | 44 ± 6 b,c | 117 ± 14 a,c | 32 ± 3 a,b |
RAD | 49 ± 4 b,c | 91 ± 11 a,c | 19 ± 10 *,a,b | |
HIPPCAM | NO RAD | 27 ± 4 b,c | 103 ± 13 a,c | 340 ± 50 a,b |
RAD | 59 ± 4 *,c | 55 ± 4 *,c | 211 ± 15 a,b |
Experimental Measurement of Specific Absorption Rate by FDTD | |||||||
---|---|---|---|---|---|---|---|
WE [g] | PTR [W] | E [V/m] | Time Elapsed until Perfusion [h] | SARE IN BRAIN | SARE IN BODY | ||
Mean [W/kg] | Peak 1 g | Mean [W/kg] | Peak 1 g | ||||
227.10 | 3.00 | 40.28 | Single exposure 1.5 | 0.069420 | 0.085499 | 0.040445 | 0.199947 |
219.90 | Single exposure 24 | 0.071551 | 0.088124 | 0.041687 | 0.206086 | ||
279.20 | Repeated exposure 1.5 | 0.07509 | 0.09249 | 0.04375 | 0.21629 |
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Othman, H.; López-Furelos, A.; Leiro-Vidal, J.M.; Ammari, M.; Sakly, M.; Abdelmelek, H.; Salas-Sánchez, A.Á.; Ares-Pena, F.; López-Martín, E. Exposure to 2.45 GHz Radiation Triggers Changes in HSP-70, Glucocorticoid Receptors and GFAP Biomarkers in Rat Brain. Int. J. Mol. Sci. 2021, 22, 5103. https://doi.org/10.3390/ijms22105103
Othman H, López-Furelos A, Leiro-Vidal JM, Ammari M, Sakly M, Abdelmelek H, Salas-Sánchez AÁ, Ares-Pena F, López-Martín E. Exposure to 2.45 GHz Radiation Triggers Changes in HSP-70, Glucocorticoid Receptors and GFAP Biomarkers in Rat Brain. International Journal of Molecular Sciences. 2021; 22(10):5103. https://doi.org/10.3390/ijms22105103
Chicago/Turabian StyleOthman, Haifa, Alberto López-Furelos, José Manuel Leiro-Vidal, Mohamed Ammari, Mohsen Sakly, Hafedh Abdelmelek, Aarón Ángel Salas-Sánchez, Francisco Ares-Pena, and Elena López-Martín. 2021. "Exposure to 2.45 GHz Radiation Triggers Changes in HSP-70, Glucocorticoid Receptors and GFAP Biomarkers in Rat Brain" International Journal of Molecular Sciences 22, no. 10: 5103. https://doi.org/10.3390/ijms22105103