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Article

Adult Renal Stem/Progenitor Cells Can Modulate T Regulatory Cells and Double Negative T Cells

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Nephrology, Dialysis and Transplantation Unit, DETO, University of Bari “Aldo Moro”, 70124 Bari, Italy
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Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari “Aldo Moro”, 70124 Bari, Italy
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Allergology Unit, DETO, University of Bari “Aldo Moro”, 70124 Bari, Italy
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Institutional BioBank, Experimental Oncology and Biobank Management Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, Italy
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Nephrology, Dialysis and Transplantation Unit, Department of Medical and Surgical Science, University of Foggia, 71122 Foggia, Italy
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Urology Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, Italy
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Department of Interdisciplinary Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy
*
Author to whom correspondence should be addressed.
C. Curci and A. Picerno equally contributed to the present study.
Loreto Gesualdo and Fabio Sallustio share senior authorship.
Int. J. Mol. Sci. 2021, 22(1), 274; https://doi.org/10.3390/ijms22010274
Received: 20 November 2020 / Revised: 22 December 2020 / Accepted: 25 December 2020 / Published: 29 December 2020
(This article belongs to the Section Molecular Immunology)
Adult Renal Stem/Progenitor Cells (ARPCs) have been recently identified in the human kidney and several studies show their active role in kidney repair processes during acute or chronic injury. However, little is known about their immunomodulatory properties and their capacity to regulate specific T cell subpopulations. We co-cultured ARPCs activated by triggering Toll-Like Receptor 2 (TLR2) with human peripheral blood mononuclear cells for 5 days and 15 days and studied their immunomodulatory capacity on T cell subpopulations. We found that activated-ARPCs were able to decrease T cell proliferation but did not affect CD8+ and CD4+ T cells. Instead, Tregs and CD3+ CD4- CD8- double-negative (DN) T cells decreased after 5 days and increased after 15 days of co-culture. In addition, we found that PAI1, MCP1, GM-CSF, and CXCL1 were significantly expressed by TLR2-activated ARPCs alone and were up-regulated in T cells co-cultured with activated ARPCs. The exogenous cocktail of cytokines was able to reproduce the immunomodulatory effects of the co-culture with activated ARPCs. These data showed that ARPCs can regulate immune response by inducing Tregs and DN T cells cell modulation, which are involved in the balance between immune tolerance and autoimmunity. View Full-Text
Keywords: stem cells; immunomodulation; tregs; DN T cells; renal diseases stem cells; immunomodulation; tregs; DN T cells; renal diseases
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MDPI and ACS Style

Curci, C.; Picerno, A.; Chaoul, N.; Stasi, A.; De Palma, G.; Franzin, R.; Pontrelli, P.; Castellano, G.; Pertosa, G.B.; Macchia, L.; Di Lorenzo, V.F.; Sabbà, C.; Gallone, A.; Gesualdo, L.; Sallustio, F. Adult Renal Stem/Progenitor Cells Can Modulate T Regulatory Cells and Double Negative T Cells. Int. J. Mol. Sci. 2021, 22, 274. https://doi.org/10.3390/ijms22010274

AMA Style

Curci C, Picerno A, Chaoul N, Stasi A, De Palma G, Franzin R, Pontrelli P, Castellano G, Pertosa GB, Macchia L, Di Lorenzo VF, Sabbà C, Gallone A, Gesualdo L, Sallustio F. Adult Renal Stem/Progenitor Cells Can Modulate T Regulatory Cells and Double Negative T Cells. International Journal of Molecular Sciences. 2021; 22(1):274. https://doi.org/10.3390/ijms22010274

Chicago/Turabian Style

Curci, Claudia, Angela Picerno, Nada Chaoul, Alessandra Stasi, Giuseppe De Palma, Rossana Franzin, Paola Pontrelli, Giuseppe Castellano, Giovanni B. Pertosa, Luigi Macchia, Vito Francesco Di Lorenzo, Carlo Sabbà, Anna Gallone, Loreto Gesualdo, and Fabio Sallustio. 2021. "Adult Renal Stem/Progenitor Cells Can Modulate T Regulatory Cells and Double Negative T Cells" International Journal of Molecular Sciences 22, no. 1: 274. https://doi.org/10.3390/ijms22010274

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