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Article

Cell-Type Specific Metabolic Response of Cancer Cells to Curcumin

1
Laboratory for Epigenomics, Ruđer Bošković Institute, Division of Molecular Medicine, 10 000 Zagreb, Croatia
2
Laboratory for Oxidative Stress (LabOS), Ruđer Bošković Institute, Division of Molecular Medicine, 10 000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(5), 1661; https://doi.org/10.3390/ijms21051661
Received: 1 February 2020 / Revised: 21 February 2020 / Accepted: 24 February 2020 / Published: 28 February 2020
(This article belongs to the Special Issue Curcumin in Health and Disease: New Knowledge)
In order to support uncontrolled proliferation, cancer cells need to adapt to increased energetic and biosynthetic requirements. One such adjustment is aerobic glycolysis or the Warburg effect. It is characterized by increased glucose uptake and lactate production. Curcumin, a natural compound, has been shown to interact with multiple molecules and signaling pathways in cancer cells, including those relevant for cell metabolism. The effect of curcumin and its solvent, ethanol, was explored on four different cancer cell lines, in which the Warburg effect varied. Vital cellular parameters (proliferation, viability) were measured along with the glucose consumption and lactate production. The transcripts of pyruvate kinase 1 and 2 (PKM1, PKM2), serine hydroxymethyltransferase 2 (SHMT2) and phosphoglycerate dehydrogenase (PHGDH) were quantified with RT-qPCR. The amount and intracellular localization of PKM1, PKM2 and signal transducer and activator of transcription 3 (STAT3) proteins were analyzed by Western blot. The response to ethanol and curcumin seemed to be cell-type specific, with respect to all parameters analyzed. High sensitivity to curcumin was present in the cell lines originating from head and neck squamous cell carcinomas: FaDu, Detroit 562 and, especially, Cal27. Very low sensitivity was observed in the colon adenocarcinoma-originating HT-29 cell line, which retained, after exposure to curcumin, a higher levels of lactate production despite decreased glucose consumption. The effects of ethanol were significant. View Full-Text
Keywords: PKM1; PKM2; SHMT2; PHGDH; STAT3; serine; intracellular localization; ethanol PKM1; PKM2; SHMT2; PHGDH; STAT3; serine; intracellular localization; ethanol
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MDPI and ACS Style

Mojzeš, A.; Tomljanović, M.; Milković, L.; Novak Kujundžić, R.; Čipak Gašparović, A.; Gall Trošelj, K. Cell-Type Specific Metabolic Response of Cancer Cells to Curcumin. Int. J. Mol. Sci. 2020, 21, 1661. https://doi.org/10.3390/ijms21051661

AMA Style

Mojzeš A, Tomljanović M, Milković L, Novak Kujundžić R, Čipak Gašparović A, Gall Trošelj K. Cell-Type Specific Metabolic Response of Cancer Cells to Curcumin. International Journal of Molecular Sciences. 2020; 21(5):1661. https://doi.org/10.3390/ijms21051661

Chicago/Turabian Style

Mojzeš, Anamarija, Marko Tomljanović, Lidija Milković, Renata Novak Kujundžić, Ana Čipak Gašparović, and Koraljka Gall Trošelj. 2020. "Cell-Type Specific Metabolic Response of Cancer Cells to Curcumin" International Journal of Molecular Sciences 21, no. 5: 1661. https://doi.org/10.3390/ijms21051661

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