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Open AccessArticle

Identification of Novel ARSB Genes Necessary for p-Benzoquinone Biosynthesis in the Larval Oral Secretion Participating in External Immune Defense in the Red Palm Weevil

1
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
2
Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(5), 1610; https://doi.org/10.3390/ijms21051610
Received: 30 January 2020 / Revised: 16 February 2020 / Accepted: 24 February 2020 / Published: 26 February 2020
(This article belongs to the Special Issue Molecular Ecology, Physiology and Biochemistry of Insects 2.0)
External secretions, composed of a variety of chemical components, are among the most important traits that endow insects with the ability to defend themselves against predators, parasites, or other adversities, especially pathogens. Thus, these exudates play a crucial role in external immunity. Red palm weevil larvae are prolific in this regard, producing large quantities of p-benzoquinone, which is present in their oral secretion. Benzoquinone with antimicrobial activity has been proven to be an active ingredient and key factor for external immunity in a previous study. To obtain a better understanding of the genetic and molecular basis of external immune secretions, we identify genes necessary for p-benzoquinone synthesis. Three novel ARSB genes, namely, RfARSB-0311, RfARSB-11581, and RfARSB-14322, are screened, isolated, and molecularly characterized on the basis of transcriptome data. To determine whether these genes are highly and specifically expressed in the secretory gland, we perform tissue/organ-specific expression profile analysis. The functions of these genes are further determined by examining the antimicrobial activity of the secretions and quantification of p-benzoquinone after RNAi. All the results reveal that the ARSB gene family can regulate the secretory volume of p-benzoquinone by participating in the biosynthesis of quinones, thus altering the host’s external immune inhibitory efficiency. View Full-Text
Keywords: arylsulfatase B; external immune secretion; gene expression profile; inhibitory efficiency; p-benzoquinone; Rhynchophorus ferrugineus; salivary gland arylsulfatase B; external immune secretion; gene expression profile; inhibitory efficiency; p-benzoquinone; Rhynchophorus ferrugineus; salivary gland
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Pu, Y.-C.; Liang, X.-Y.; Zhang, H.; Zhang, H.-J.; Xu, L.-N.; Ji, Y.-N.; Huang, S.-N.; Bai, J.; Hou, Y.-M. Identification of Novel ARSB Genes Necessary for p-Benzoquinone Biosynthesis in the Larval Oral Secretion Participating in External Immune Defense in the Red Palm Weevil. Int. J. Mol. Sci. 2020, 21, 1610.

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