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Open AccessArticle

Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow

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Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941902, Brazil
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Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941902, Brazil
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Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 2268501, Japan
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National Center of Structural Biology and Bioimaging, Federal University of Rio de Janeiro, Rio de Janeiro 21941902, Brazil
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National Institute of Science and Technology for Regenerative Medicine-REGENERA, Federal University of Rio de Janeiro, Rio de Janeiro 21941902, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(24), 9563; https://doi.org/10.3390/ijms21249563
Received: 25 October 2020 / Revised: 27 November 2020 / Accepted: 10 December 2020 / Published: 15 December 2020
Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Therefore, the aim of this study was to evaluate the angiogenic properties of menstrual blood-derived mesenchymal stromal cells (mbMSC) in vitro and in vivo and compared to bmMSC. MSC’s intrinsic angiogenic capacity was assessed by sprouting and migration assays. mbMSC presented higher invasion and longer sprouts in 3D culture. Additionally, both MSC-spheroids showed cells expressing CD31. mbMSC and bmMSC were able to migrate after scratch wound in vitro, nonetheless, only mbMSC demonstrated ability to engraft in the chick embryo, migrating to perivascular, perineural, and chondrogenic regions. In order to study the paracrine effects, mbMSC and bmMSC conditioned mediums were capable of stimulating HUVEC’s tube-like formation and migration. Both cells expressed VEGF-A and FGF2. Meanwhile, PDGF-B was expressed exclusively in mbMSC. Our results indicated that mbMSC and bmMSC presented a promising angiogenic potential. However, mbMSC seems to have additional advantages since it can be obtained by non-invasive procedure and expresses PDGF-B, an important molecule for vascular formation and remodeling. View Full-Text
Keywords: menstrual blood-derived mesenchymal stromal cells; bone marrow mesenchymal stromal cells; human umbilical vein endothelial cells; angiogenesis menstrual blood-derived mesenchymal stromal cells; bone marrow mesenchymal stromal cells; human umbilical vein endothelial cells; angiogenesis
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MDPI and ACS Style

Santos, R.d.A.; Asensi, K.D.; de Barros, J.H.O.; de Menezes, R.C.S.; Cordeiro, I.R.; Neto, J.M.d.B.; Kasai-Brunswick, T.H.; Goldenberg, R.C.d.S. Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow. Int. J. Mol. Sci. 2020, 21, 9563. https://doi.org/10.3390/ijms21249563

AMA Style

Santos RdA, Asensi KD, de Barros JHO, de Menezes RCS, Cordeiro IR, Neto JMdB, Kasai-Brunswick TH, Goldenberg RCdS. Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow. International Journal of Molecular Sciences. 2020; 21(24):9563. https://doi.org/10.3390/ijms21249563

Chicago/Turabian Style

Santos, Rosana d.A.; Asensi, Karina D.; de Barros, Julia H.O.; de Menezes, Rafael C.S.; Cordeiro, Ingrid R.; Neto, José M.d.B.; Kasai-Brunswick, Tais H.; Goldenberg, Regina C.d.S. 2020. "Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow" Int. J. Mol. Sci. 21, no. 24: 9563. https://doi.org/10.3390/ijms21249563

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