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Article

Development of an Organ-on-a-Chip-Device for Study of Placental Pathologies

1
Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431, USA
2
Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA
3
Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL 33431, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8755; https://doi.org/10.3390/ijms21228755
Received: 29 September 2020 / Revised: 1 November 2020 / Accepted: 17 November 2020 / Published: 19 November 2020
(This article belongs to the Section Molecular Endocrinology and Metabolism)
The human placenta plays a key role in reproduction and serves as a major interface for maternofetal exchange of nutrients. Study of human placenta pathology presents a great experimental challenge because it is not easily accessible. In this paper, a 3D placenta-on-a-chip model is developed by bioengineering techniques to simulate the placental interface between maternal and fetal blood in vitro. In this model, trophoblasts cells and human umbilical vein endothelial cells are cultured on the opposite sides of a porous polycarbonate membrane, which is sandwiched between two microfluidic channels. Glucose diffusion across this barrier is analyzed under shear flow conditions. Meanwhile, a numerical model of the 3D placenta-on-a-chip model is developed. Numerical results of concentration distributions and the convection–diffusion mass transport is compared to the results obtained from the experiments for validation. Finally, effects of flow rate and membrane porosity on glucose diffusion across the placental barrier are studied using the validated numerical model. The placental model developed here provides a potentially helpful tool to study a variety of other processes at the maternal–fetal interface, for example, effects of drugs or infections like malaria on transport of various substances across the placental barrier. View Full-Text
Keywords: placenta-on-a-chip; glucose transport; molecular concentration distribution; microfluidics placenta-on-a-chip; glucose transport; molecular concentration distribution; microfluidics
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MDPI and ACS Style

Mosavati, B.; Oleinikov, A.V.; Du, E. Development of an Organ-on-a-Chip-Device for Study of Placental Pathologies. Int. J. Mol. Sci. 2020, 21, 8755. https://doi.org/10.3390/ijms21228755

AMA Style

Mosavati B, Oleinikov AV, Du E. Development of an Organ-on-a-Chip-Device for Study of Placental Pathologies. International Journal of Molecular Sciences. 2020; 21(22):8755. https://doi.org/10.3390/ijms21228755

Chicago/Turabian Style

Mosavati, Babak, Andrew V. Oleinikov, and E. Du 2020. "Development of an Organ-on-a-Chip-Device for Study of Placental Pathologies" International Journal of Molecular Sciences 21, no. 22: 8755. https://doi.org/10.3390/ijms21228755

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