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Open AccessArticle

Triplex Real-Time PCR Approach for the Detection of Crucial Fungal Berry Pathogens—Botrytis spp., Colletotrichum spp. and Verticillium spp.

Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
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Int. J. Mol. Sci. 2020, 21(22), 8469; https://doi.org/10.3390/ijms21228469
Received: 30 September 2020 / Revised: 4 November 2020 / Accepted: 8 November 2020 / Published: 11 November 2020
(This article belongs to the Section Molecular Plant Sciences)
Phytopathogens cause undeniably serious damage in agriculture by harming fruit cultivations and lowering harvest yields, which as a consequence substantially reduces food production efficiency. Fungi of the Botrytis, Colletotrichum and Verticillium genera are a main concern in berry production. However, no rapid detection method for detecting all of these pathogens simultaneously has been developed to date. Therefore, in this study, a multiplex real-time PCR assay for this purpose was established. Universal fungal primers for the D2 region of the large subunit ribosomal DNA and three multiplexable fluorogenic probes specific for the chosen fungi were designed and deployed. The triplex approach for the molecular detection of these fungi, which was developed in this study, allows for the rapid and effective detection of crucial berry pathogens, which contributes to a more rapid implementation of protective measures in plantations and a significant reduction in losses caused by fungal diseases. View Full-Text
Keywords: D2LSU; Botrytis spp.; Colletotrichum spp.; Verticillium spp.; real-time PCR; strawberry pathogens; rapid detection D2LSU; Botrytis spp.; Colletotrichum spp.; Verticillium spp.; real-time PCR; strawberry pathogens; rapid detection
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MDPI and ACS Style

Malarczyk, D.G.; Panek, J.; Frąc, M. Triplex Real-Time PCR Approach for the Detection of Crucial Fungal Berry Pathogens—Botrytis spp., Colletotrichum spp. and Verticillium spp. Int. J. Mol. Sci. 2020, 21, 8469. https://doi.org/10.3390/ijms21228469

AMA Style

Malarczyk DG, Panek J, Frąc M. Triplex Real-Time PCR Approach for the Detection of Crucial Fungal Berry Pathogens—Botrytis spp., Colletotrichum spp. and Verticillium spp. International Journal of Molecular Sciences. 2020; 21(22):8469. https://doi.org/10.3390/ijms21228469

Chicago/Turabian Style

Malarczyk, Dominika G.; Panek, Jacek; Frąc, Magdalena. 2020. "Triplex Real-Time PCR Approach for the Detection of Crucial Fungal Berry Pathogens—Botrytis spp., Colletotrichum spp. and Verticillium spp." Int. J. Mol. Sci. 21, no. 22: 8469. https://doi.org/10.3390/ijms21228469

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