Stress-Specific Spatiotemporal Responses of RNA-Binding Proteins in Human Stem Cell-Derived Motor Neurons
Abstract
:1. Introduction
2. Results
2.1. RBPs Reveal Distinct Nucleocytoplasmic Distributions in Human MNs
2.2. Osmotic Stress Causes RBP-Specific Changes in Nucleocytoplasmic Distribution
2.3. RBP Nucleocytoplasmic Redistribution is Stress Specific
2.4. TDP-43 and FUS Exhibit Slower Nuclear Relocalisation Dynamics Following Stress
2.5. ALS-Causing VCP Mutations Do Not Perturb Nuclear Relocalisation Dynamics of TDP-43 and FUS Following Stress
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Stress Treatments
4.3. Immunocytochemistry
4.4. Image Acquisition and Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Harley, J.; Patani, R. Stress-Specific Spatiotemporal Responses of RNA-Binding Proteins in Human Stem Cell-Derived Motor Neurons. Int. J. Mol. Sci. 2020, 21, 8346. https://doi.org/10.3390/ijms21218346
Harley J, Patani R. Stress-Specific Spatiotemporal Responses of RNA-Binding Proteins in Human Stem Cell-Derived Motor Neurons. International Journal of Molecular Sciences. 2020; 21(21):8346. https://doi.org/10.3390/ijms21218346
Chicago/Turabian StyleHarley, Jasmine, and Rickie Patani. 2020. "Stress-Specific Spatiotemporal Responses of RNA-Binding Proteins in Human Stem Cell-Derived Motor Neurons" International Journal of Molecular Sciences 21, no. 21: 8346. https://doi.org/10.3390/ijms21218346