Cyclin Y Is Expressed in Platelets and Modulates Integrin Outside-in Signaling
Abstract
:1. Introduction
2. Results
2.1. Cyclin Y Is Expressed in Platelets and Is Ubiquitinated in Response to Platelet Stimulation by Thrombin
2.2. Cyclin Y Is Involved in Platelet Biogenesis and Function
2.3. CCNY Regulates β3 Integrin Tyrosine Phosphorylation
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Study Subjects
4.3. Animals
4.4. Blood Counting
4.5. Flow Cytometry
4.6. Platelet Isolation
4.7. Platelet Aggregation
4.8. ATP Assay
4.9. P-selectin Expression
4.10. Platelet Adhesion and Spreading Assays
4.11. Clot Retraction
4.12. Bleeding Time
4.13. LC-MS-based Proteomics
4.14. Proteomic Data Analysis
4.15. Activation of Calpain In Vitro
4.16. Western Blotting
4.17. Immunoprecipitation
4.18. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Kyselova, A.; Siragusa, M.; Anthes, J.; Solari, F.A.; Loroch, S.; Zahedi, R.P.; Walter, U.; Fleming, I.; Randriamboavonjy, V. Cyclin Y Is Expressed in Platelets and Modulates Integrin Outside-in Signaling. Int. J. Mol. Sci. 2020, 21, 8239. https://doi.org/10.3390/ijms21218239
Kyselova A, Siragusa M, Anthes J, Solari FA, Loroch S, Zahedi RP, Walter U, Fleming I, Randriamboavonjy V. Cyclin Y Is Expressed in Platelets and Modulates Integrin Outside-in Signaling. International Journal of Molecular Sciences. 2020; 21(21):8239. https://doi.org/10.3390/ijms21218239
Chicago/Turabian StyleKyselova, Anastasia, Mauro Siragusa, Julian Anthes, Fiorella Andrea Solari, Stefan Loroch, René P. Zahedi, Ulrich Walter, Ingrid Fleming, and Voahanginirina Randriamboavonjy. 2020. "Cyclin Y Is Expressed in Platelets and Modulates Integrin Outside-in Signaling" International Journal of Molecular Sciences 21, no. 21: 8239. https://doi.org/10.3390/ijms21218239