Comparative Ubiquitome Analysis under Heat Stress Reveals Diverse Functions of Ubiquitination in Saccharina japonica
Abstract
:1. Introduction
2. Results
2.1. Overview of the Ubiquitomic Analysis
2.2. Properties of Ubiquitination Sites and Motifs
2.3. Gene Ontology (GO) Functional Classification and GO Enrichment-Based Clustering Analysis of the Differentially Expressed Ubiquitinated Proteins
2.4. Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analysis of the Differentially Expressed Ubiquitinated Proteins
2.5. Protein Domain Based Enrichment Analysis of the Differentially Expressed Lysine Ubiquitinated Proteins
2.6. Protein–Protein Interaction (PPI) Network Analysis of Differentially Expressed Ubiquitinated Proteins
3. Discussion
3.1. Ubiquitination Involved in the Heat Response of S. japonica
3.2. The UPS-Related Protein Showed Differential Ubiquitination
3.3. Key Biological Processes are Modified by Ubiquitination under Heat Stress
4. Materials and Methods
4.1. Sample Preparation
4.2. Protein Extraction
4.3. Trypsin Digestion
4.4. High Performance Liquid Chromatography (HPLC) Fractionation
4.5. Affinity Enrichment of Ubiquitinated Peptides
4.6. Liquid Chromatography (LC)-Tandem Mass Spectroscopy (MS/MS) Analysis
4.7. Database Search and Quantification
4.8. Bioinformatics Methods
4.9. Western Blot Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Name | Upregulated (>1.5) | Downregulated (<0.67) |
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Sites | 152 | 208 |
Proteins | 106 | 131 |
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Pengyan, Z.; Fuli, L.; Siqing, C.; Zhourui, L.; Wenjun, W.; Xiutao, S. Comparative Ubiquitome Analysis under Heat Stress Reveals Diverse Functions of Ubiquitination in Saccharina japonica. Int. J. Mol. Sci. 2020, 21, 8210. https://doi.org/10.3390/ijms21218210
Pengyan Z, Fuli L, Siqing C, Zhourui L, Wenjun W, Xiutao S. Comparative Ubiquitome Analysis under Heat Stress Reveals Diverse Functions of Ubiquitination in Saccharina japonica. International Journal of Molecular Sciences. 2020; 21(21):8210. https://doi.org/10.3390/ijms21218210
Chicago/Turabian StylePengyan, Zhang, Liu Fuli, Chen Siqing, Liang Zhourui, Wang Wenjun, and Sun Xiutao. 2020. "Comparative Ubiquitome Analysis under Heat Stress Reveals Diverse Functions of Ubiquitination in Saccharina japonica" International Journal of Molecular Sciences 21, no. 21: 8210. https://doi.org/10.3390/ijms21218210