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Article

Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion

1
Department of Integrative Biosciences, University of Brain Education (UBE), Cheonan 31228, Korea
2
Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea
3
Division of Bio-Medical Science & Technology (Biomedical Engineering), KIST School, Korea University of Science and Technology (UST), Seoul 02792, Korea
4
Department of Biomedical Engineering, Chung-Ang University, Seoul 06974, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(21), 8153; https://doi.org/10.3390/ijms21218153
Received: 31 August 2020 / Revised: 20 October 2020 / Accepted: 28 October 2020 / Published: 31 October 2020
(This article belongs to the Collection Feature Papers in Molecular Oncology)
Cancer-associated fibroblasts (CAFs) in the cancer microenvironment play an essential role in metastasis. Differentiation of endothelial cells into CAFs is induced by cancer cell-derived exosomes secreted from cancer cells that transfer molecular signals to surrounding cells. Differentiated CAFs facilitate migration of cancer cells to different regions through promoting extracellular matrix (ECM) modifications. However, in vitro models in which endothelial cells exposed to cancer cell-derived exosomes secreted from various cancer cell types differentiate into CAFs or a microenvironmentally controlled model for investigating cancer cell invasion by CAFs have not yet been studied. In this study, we propose a three-dimensional in vitro cancer cell invasion model for real-time monitoring of the process of forming a cancer invasion site through CAFs induced by exosomes isolated from three types of cancer cell lines. The invasiveness of cancer cells with CAFs induced by cancer cell-derived exosomes (eCAFs) was significantly higher than that of CAFs induced by cancer cells (cCAFs) through physiological and genetic manner. In addition, different genetic tendencies of the invasion process were observed in the process of invading cancer cells according to CAFs. Our 3D microfluidic platform helps to identify specific interactions among multiple factors within the cancer microenvironment and provides a model for cancer drug development. View Full-Text
Keywords: cancer-associated fibroblasts; cancer cell-derived exosomes; invasive cancer cells; cancer cell invasion; 3D microfluidics cancer-associated fibroblasts; cancer cell-derived exosomes; invasive cancer cells; cancer cell invasion; 3D microfluidics
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MDPI and ACS Style

Kim, K.; Sohn, Y.J.; Lee, R.; Yoo, H.J.; Kang, J.Y.; Choi, N.; Na, D.; Yeon, J.H. Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion. Int. J. Mol. Sci. 2020, 21, 8153. https://doi.org/10.3390/ijms21218153

AMA Style

Kim K, Sohn YJ, Lee R, Yoo HJ, Kang JY, Choi N, Na D, Yeon JH. Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion. International Journal of Molecular Sciences. 2020; 21(21):8153. https://doi.org/10.3390/ijms21218153

Chicago/Turabian Style

Kim, Kimin, Yeh Joo Sohn, Ruri Lee, Hye Ju Yoo, Ji Yoon Kang, Nakwon Choi, Dokyun Na, and Ju Hun Yeon. 2020. "Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion" International Journal of Molecular Sciences 21, no. 21: 8153. https://doi.org/10.3390/ijms21218153

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