p53 Immunohistochemical Patterns in HPV-Independent Squamous Cell Carcinomas of the Vulva and the Associated Skin Lesions: A Study of 779 Cases
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Case Selection
4.2. Tissue Preparation, Nucleic-Acid Isolation, and Human Papillomavirus (HPV) DNA Detection
4.3. Histological Evaluation
4.4. p53 Immunohistochemical (IHC)
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Pattern of p53 Expression | ||||||
---|---|---|---|---|---|---|
Abnormal Patterns | Wild-Type Patterns | |||||
Adjacent Skin Lesion | Basal Overexpression | Parabasal/Diffuse Overexpression | Null | Cytoplasmic | Scattered | Mid-Epithelial |
Intraepithelial precursors | ||||||
dVIN (n = 186) | 48 (25.8%) | 67 (36.0%) | 13 (7.0%) | 1 (0.5%) | 57 (30.7%) | 0 (0%) |
HSIL-like lesion (n = 46) | 2 (4.3%) | 27 (58.7%) | 4 (8.7%) | 1 (2.2%) | 11 (23.9%) | 1 (2.2%) |
VAAD/DEVIL (n = 22) | 5 (22.7%) | 4 (18.2%) | 0 (0%) | 0 (0%) | 13 (59.1%) | 0 (0%) |
Inflammatory lesions | ||||||
Lichen sclerosus (n = 36) | 6 (16.7%) | 0 0%) | 3 (8.3%) | 0 (0%) | 27 (75.0%) | 0 (0%) |
Other inflammatory/reactive lesions (n = 160) | 14 (8.7%) | 15 (9.4%) | 0 (0%) | 0 (0%) | 131 (81.9%) | 0 (0%) |
Total | 75 | 113 | 20 | 2 | 239 | 1 |
Pattern of p53 Expression in the Adjacent Skin Lesion | ||||||
---|---|---|---|---|---|---|
Abnormal Patterns (n = 210) | Wild-Type Patterns (n = 240) | |||||
Pattern of p53 Expression in the Invasive Carcinoma | Basal Overexpression | Parabasal/Diffuse Overexpression | Null | Cytoplasmic | Scattered | Mid-Epithelial |
Abnormal patterns (n = 306) | ||||||
Basal overexpression (n = 84) | 39 (46.4%) | 6 (7.2%) | 0 (0%) | 0 (0%) | 39 (46.4%) | 0 (0%) |
Parabasal/Diffuse overexpression (n = 193) | 36 (18.7%) | 107 (55.4%) | 0 (0%) | 0 (0%) | 50 (25.9%) | 0 (0%) |
Null (n = 27) | 0 (0%) | 0 (0%) | 20 (74.1%) | 0 (0%) | 7 (25.9%) | 0 (0%) |
Cytoplasmic (n = 2) | 0 (0%) | 0 (0%) | 0 (0%) | 2 (100%) | 0 (0%) | 0 (0%) |
Wild-type patterns (n = 144) | ||||||
Scattered (n = 143) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 143 (100%) | 0 (0%) |
Mid-epithelial (n = 1) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 1 (100%) |
Total | 75 | 113 | 20 | 2 | 239 | 1 |
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Rakislova, N.; Alemany, L.; Clavero, O.; Saco, A.; Torné, A.; del Pino, M.; Munmany, M.; Rodrigo-Calvo, M.T.; Guerrero, J.; Marimon, L.; et al. p53 Immunohistochemical Patterns in HPV-Independent Squamous Cell Carcinomas of the Vulva and the Associated Skin Lesions: A Study of 779 Cases. Int. J. Mol. Sci. 2020, 21, 8091. https://doi.org/10.3390/ijms21218091
Rakislova N, Alemany L, Clavero O, Saco A, Torné A, del Pino M, Munmany M, Rodrigo-Calvo MT, Guerrero J, Marimon L, et al. p53 Immunohistochemical Patterns in HPV-Independent Squamous Cell Carcinomas of the Vulva and the Associated Skin Lesions: A Study of 779 Cases. International Journal of Molecular Sciences. 2020; 21(21):8091. https://doi.org/10.3390/ijms21218091
Chicago/Turabian StyleRakislova, Natalia, Laia Alemany, Omar Clavero, Adela Saco, Aureli Torné, Marta del Pino, Meritxell Munmany, Maria Teresa Rodrigo-Calvo, José Guerrero, Lorena Marimon, and et al. 2020. "p53 Immunohistochemical Patterns in HPV-Independent Squamous Cell Carcinomas of the Vulva and the Associated Skin Lesions: A Study of 779 Cases" International Journal of Molecular Sciences 21, no. 21: 8091. https://doi.org/10.3390/ijms21218091