Conjugation with Methylsulfonylmethane Improves Hyaluronic Acid Anti-Inflammatory Activity in a Hydrogen Peroxide-Exposed Tenocyte Culture In Vitro Model
Abstract
:1. Introduction
2. Results
2.1. Cell Morphology and Proliferation
2.2. Lactate Dehydrogenase (LDH) Cytotoxicity Assay and Protein Quantification
2.3. iNOS Expression
2.4. PGE2 Secretion
2.5. Metalloproteinases and Expression of Collagen Proteins
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Treatment
4.3. H2O2 Treatment
4.4. MTT Assay for cell Proliferation
4.5. Lactate Dehydrogenase (LDH) Release Assay
4.6. Cell Lysis and Protein Extraction
4.7. Prostaglandin E2 Secretion
4.8. Western Blot Analysis
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Commercial Name | Active Substance | Molecular Weight | Source | Doses Tested | Manufacturer |
---|---|---|---|---|---|
Artrosulfur® HA | Linear Sodium Hyaluronate | 1200 KDa | Bacterial Fermentation | 1000 ug/mL | Alfakjn S.r.l., Garlasco (PV) Italy |
MSM | Methylsulfonylmethane | / | Oxidation of DMSO with hydrogen (H2O2) and purified by distillation | 3125 ug/mL | Barentz, Paderno Dugnano (MI), Italy |
Artrosulfur® HA + HA | Linear Sodium Hyaluronate + Methylsulfonylmethane (5% w/v) | 1200 KDa | Bacterial Fermentation | 1000 ug/mL + 3125 ug/mL | Alfakjn S.r.l., Garlasco (PV) Italy |
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Oliva, F.; Gallorini, M.; Antonetti Lamorgese Passeri, C.; Gissi, C.; Ricci, A.; Cataldi, A.; Colosimo, A.; Berardi, A.C. Conjugation with Methylsulfonylmethane Improves Hyaluronic Acid Anti-Inflammatory Activity in a Hydrogen Peroxide-Exposed Tenocyte Culture In Vitro Model. Int. J. Mol. Sci. 2020, 21, 7956. https://doi.org/10.3390/ijms21217956
Oliva F, Gallorini M, Antonetti Lamorgese Passeri C, Gissi C, Ricci A, Cataldi A, Colosimo A, Berardi AC. Conjugation with Methylsulfonylmethane Improves Hyaluronic Acid Anti-Inflammatory Activity in a Hydrogen Peroxide-Exposed Tenocyte Culture In Vitro Model. International Journal of Molecular Sciences. 2020; 21(21):7956. https://doi.org/10.3390/ijms21217956
Chicago/Turabian StyleOliva, Francesco, Marialucia Gallorini, Cristina Antonetti Lamorgese Passeri, Clarissa Gissi, Alessia Ricci, Amelia Cataldi, Alessia Colosimo, and Anna Concetta Berardi. 2020. "Conjugation with Methylsulfonylmethane Improves Hyaluronic Acid Anti-Inflammatory Activity in a Hydrogen Peroxide-Exposed Tenocyte Culture In Vitro Model" International Journal of Molecular Sciences 21, no. 21: 7956. https://doi.org/10.3390/ijms21217956