Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases
Abstract
:1. Why Using Defined Chitosans Is so All-Important in Bioactivity Studies
2. Regio-Selective Chitin de-N-Acteylases
3. Preparation of the Substrates (An and Dn)
4. paCOS Nomenclature
5. Preparation of paCOS Dimers
6. Preparation of paCOS Trimers
7. Preparation of paCOS Tetramers
8. Preparation of paCOS Pentamers and Hexamers
9. Mass Spectrometric Analysis of paCOS
10. Chromatographic Purification of paCOS
11. Conclusions and Outlook
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
DP | Degree of Polymerisation |
FA | Fraction of Acetylation |
PA | Pattern of Acetylation |
paCOS | partially acetylated Chitosan Oligosaccharides |
CDA | Chitin Deacetylase |
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Enzyme | Step | Substrate | Reference | ||||
---|---|---|---|---|---|---|---|
AA | AAA | AAAA | AAAAA | AAAAAA | |||
NodB | 1 | DA | DAA | DAAA | DAAAA | DAAAAA | [35] |
VcCDA | 1 | AD | ADA | ADAA | ADAAA | ADAAAA | [36] |
CnCDA4 | 1 | - | (ADA) | AADA | AAADA | AAAADA | [27] |
2 | - | AADD | AADDA | AAADDA | |||
3 | - | - | AADDDA | ||||
PesCDA | 1 | - | - | AADA | AA[AD]A | AA[A2D1]A | [37] |
2 | - | AADDA | AA[A1D2]A | ||||
3 | - | AADDDA | |||||
PgtCDA | 1 | p.n.d. | p.n.d. | AADA | AAADA (AADAA) | AAADAA (AADAAA) | [31] |
2 | AADD | AADDA (AAADD) | AAADDA (AADDAA) | ||||
3 | - | AADDD | AADDDA (AAADDD) | ||||
4 | - | AADDDD | |||||
PcCDA | 1 | a.n.d. | - | A3D1 | ADAAA | a.n.d. | [38] |
2 | A2D2 | ADDAA | |||||
BsPdaC-CD | 1 | - | p.n.d. | ADAA AADA | AAADA (AADAA) | a.n.d. | [39] |
2 | ADDA (DADA) | AADDA (ADADA) | |||||
3 | DDDA | ADDDA | |||||
4 | - | DDDDA |
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Bonin, M.; Sreekumar, S.; Cord-Landwehr, S.; Moerschbacher, B.M. Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases. Int. J. Mol. Sci. 2020, 21, 7835. https://doi.org/10.3390/ijms21217835
Bonin M, Sreekumar S, Cord-Landwehr S, Moerschbacher BM. Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases. International Journal of Molecular Sciences. 2020; 21(21):7835. https://doi.org/10.3390/ijms21217835
Chicago/Turabian StyleBonin, Martin, Sruthi Sreekumar, Stefan Cord-Landwehr, and Bruno M. Moerschbacher. 2020. "Preparation of Defined Chitosan Oligosaccharides Using Chitin Deacetylases" International Journal of Molecular Sciences 21, no. 21: 7835. https://doi.org/10.3390/ijms21217835