The Role of SHIP1 on Apoptosis and Autophagy in the Adipose Tissue of Obese Mice
Abstract
1. Introduction
2. Results
2.1. Macrophage Infiltration and Inflammation in the Adipose Tissue of DIO and ob/ob Mice
2.2. Differential Gene Expression in the Adipose Tissue of DIO and ob/ob Mice
2.3. Effects of DIO and Leptin Deficiency on Inflammation and Apoptosis in Adipose Tissue
2.4. Effects of DIO and Leptin Deficiency on Autophagy in Adipose Tissue
2.5. Effects of CR on SHIP1 Expression Related to Apoptosis and Autophagy in the Adipose Tissue of DIO and ob/ob Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Enzyme-Linked Immunosorbent Assay (ELISA)
4.3. RNA-seq Analysis
4.4. Real-time Reverse Transcriptase Polymerase Chain Reaction
4.5. Histological Analysis
4.6. Protein Extractions and Western Blot Analyses
4.7. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CCR5 | C-C motif receptor 5 |
CCL5 | CC chemokine ligand 5 |
CLS | Crown-like structure |
CR | Calorie restriction |
DAPI | 4′,6-Diamidino-2-phenylindole |
DIO | Diet-induced obesity |
HFD | High-fat diet |
LAMP1 | Lysosomal-associated membrane protein 1 |
PI3K | Phosphoinositide 3-kinase |
SHIP1 | Src homology domain-containing inositol 5′-phosphatase 1 |
TUNEL | Terminal deoxynucleotidyl transferase dUTP nick-end labeling |
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Jeong, J.H.; Choi, E.B.; Jang, H.M.; Ahn, Y.J.; An, H.S.; Lee, J.Y.; Park, G.; Jeong, E.A.; Shin, H.J.; Lee, J.; et al. The Role of SHIP1 on Apoptosis and Autophagy in the Adipose Tissue of Obese Mice. Int. J. Mol. Sci. 2020, 21, 7225. https://doi.org/10.3390/ijms21197225
Jeong JH, Choi EB, Jang HM, Ahn YJ, An HS, Lee JY, Park G, Jeong EA, Shin HJ, Lee J, et al. The Role of SHIP1 on Apoptosis and Autophagy in the Adipose Tissue of Obese Mice. International Journal of Molecular Sciences. 2020; 21(19):7225. https://doi.org/10.3390/ijms21197225
Chicago/Turabian StyleJeong, Jae Hun, Eun Bee Choi, Hye Min Jang, Yu Jeong Ahn, Hyeong Seok An, Jong Youl Lee, Gyeongah Park, Eun Ae Jeong, Hyun Joo Shin, Jaewoong Lee, and et al. 2020. "The Role of SHIP1 on Apoptosis and Autophagy in the Adipose Tissue of Obese Mice" International Journal of Molecular Sciences 21, no. 19: 7225. https://doi.org/10.3390/ijms21197225
APA StyleJeong, J. H., Choi, E. B., Jang, H. M., Ahn, Y. J., An, H. S., Lee, J. Y., Park, G., Jeong, E. A., Shin, H. J., Lee, J., Kim, K. E., & Roh, G. S. (2020). The Role of SHIP1 on Apoptosis and Autophagy in the Adipose Tissue of Obese Mice. International Journal of Molecular Sciences, 21(19), 7225. https://doi.org/10.3390/ijms21197225