Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood
Abstract
:1. Introduction
2. Results
2.1. Experimental Design
2.2. Post-Natal Growth of C57Bl6 Mice Was Not Affected by a Short Exposure in M16/Pyruvate Medium at 1 Cell Embryo Stage. Wild Type (WT)
2.3. The Contractile Phenotype of the Skeletal Muscle is Highly Sensitive to a Short Exposure in M16/Pyruvate Medium at 1-Cell Embryo Stage
2.4. Skeletal Muscle Hypertrophy Was Fully Abrogated for p43−/− Male Mice by a Short Exposure in M16/Pyruvate Medium at 1 Cell Embryo Stage
2.5. Skeletal Muscle Hypertrophy Was Increased in Mstn−/− Mice of Both Genders by a Short Exposure in M16/Pyruvate Medium at 1 Cell Embryo Stage
2.6. Short Exposure in M16/Pyruvate Medium at 1 Cell Embryo Stage Also Alters Contractile Features of Skeletal Muscle in p43−/− and Mstn−/− Mice
2.7. Influence of Culture Medium at 1 Cell Embryo Stage on H3K27me and H3K4ac Marks
3. Discussion
4. Materials and Methods
4.1. Animals and Ethics Statement
4.2. Embryo Production, Collection, and Treatment
4.3. Embryo Transfer and Post-Natal Growth
4.4. Gene Expression Studies
4.5. Mitochondrial Complex IV Activity
4.6. Immunostaining of Post-Translational Modifications of Histones and Fluorescence Intensity Measurements
4.7. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Bertrand-Gaday, C.; Letheule, M.; Blanchet, E.; Vernus, B.; Pessemesse, L.; Bonnet-Garnier, A.; Bonnieu, A.; Casas, F. Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood. Int. J. Mol. Sci. 2020, 21, 7203. https://doi.org/10.3390/ijms21197203
Bertrand-Gaday C, Letheule M, Blanchet E, Vernus B, Pessemesse L, Bonnet-Garnier A, Bonnieu A, Casas F. Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood. International Journal of Molecular Sciences. 2020; 21(19):7203. https://doi.org/10.3390/ijms21197203
Chicago/Turabian StyleBertrand-Gaday, Christelle, Martine Letheule, Emilie Blanchet, Barbara Vernus, Laurence Pessemesse, Amélie Bonnet-Garnier, Anne Bonnieu, and François Casas. 2020. "Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood" International Journal of Molecular Sciences 21, no. 19: 7203. https://doi.org/10.3390/ijms21197203