Next Article in Journal
Immunomodulatory Role of Microbial Surfactants, with Special Emphasis on Fish
Next Article in Special Issue
Polysulfide and Hydrogen Sulfide Ameliorate Cisplatin-Induced Nephrotoxicity and Renal Inflammation through Persulfidating STAT3 and IKKβ
Previous Article in Journal
Structure-Based Discovery of Novel Chemical Classes of Autotaxin Inhibitors
Previous Article in Special Issue
Proanthocyanidins Ameliorated Deficits of Lipid Metabolism in Type 2 Diabetes Mellitus Via Inhibiting Adipogenesis and Improving Mitochondrial Function
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 21
Issue 19
10.3390/ijms21197003
Review Report
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation

Int. J. Mol. Sci. 2020, 21(19), 7003; https://doi.org/10.3390/ijms21197003
by Jung Joo Yoon 1,2, Hyeon Kyoung Lee 1,2, Hye Yoom Kim 1,2, Byung Hyuk Han 1,2, Ho Sub Lee 1,2, Yun Jung Lee 1,2,* and Dae Gill Kang 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2020, 21(19), 7003; https://doi.org/10.3390/ijms21197003
Submission received: 2 September 2020 / Revised: 21 September 2020 / Accepted: 22 September 2020 / Published: 23 September 2020
(This article belongs to the Special Issue Phytochemicals for Diabetes and its Complication)

Round 1

Reviewer 1 Report

Yoon et al. examined the effect of Sauchinone on the Ang II-induced cell proliferation, ECMs and inflammatory components. The experiments were well conducted. However, there are some concern to reach the conclusion.

Major

  1. The authors mentioned that the results of this study are relevant to the pathogenesis of DN in the part of Introduction and Discussion. However, the overactivation of renin-angiotensin system mimicking by Ang II incubation is not a specific mechanism for DN. Thus, the reviewer thinks that the authors should more broadly discuss the pathogenesis of the mesangial proliferative disease, including IgA nephropathy or RAS activated kidney diseases.
  2. There is no information regarding the provider and dose of Ang II.

Minor

  1. In Figure 2B and 2C, are there any quantitative data? Also, “Sauchinone(uM)” may be missing in Figure 2B.
  2. In Figure 4B and 5B, the author should provide the sample size examined.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

This manuscript demonstrates that when added to in vitro cultures of human mesangial cells stimulated with ANGII, Sauchinone decreases the expression of fibrotic and inflammatory proteins.

Some minor comments:

-How was the dose of ANGII chosen?

-How were the doses of Sauchinone chosen?

-In methods:

details of primary antibodies (including catolog number and dilutions) would be helpful.

more information about cell culture would be helpful (starting from page 9 line 293), including the culture plates used and the number of cells seeded.

-On page 8 line 219, mesangial cells are not the main cause of interstitial fibrosis, but definitely contribute to glomerular sclerosis.

-These data are a great starting point to investigate the effect of Sauchinone on mesagnial cells, but the work lacks some in vivo confirmation. Is this treatment effective after the onset of disease (in all cases cells were pre-treated with Sauchinone)? And in an in vivo model of diabetes, is this treatment useful? Without this, the conclusion is a little overstated.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

The revision was well done.

Back to TopTop